在新型蚯蚓反应器中添加过量的活性污泥以有效处理果蔬废物，从而消除抗生素抗性基因。
Elimination of antibiotic resistance genes from excess activated sludge added for effective treatment of fruit and vegetable waste in a novel vermireactor.
机构信息
Graduate School of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Department of Architecture, Lu Liang University, Lishi, Shanxi 033000, China.
出版信息
Bioresour Technol. 2021 Apr;325:124695. doi: 10.1016/j.biortech.2021.124695. Epub 2021 Jan 11.
Elimination of antibiotic resistance genes (ARGs) from excess activated sludge (EAS) mixed for effective treatment of different fruit and vegetable waste (FVW) by using a novel vermireactor consisted of substrate and bed compartments was investigated. ARGs (tet G, tet M and sul 1) and mobile genetic element gene (intl 1) were targeted and, through quantitative analysis of their abundances in both the compartments and the fresh cast of earthworms, significant reductions in substrate compartments were confirmed for the treatments for FVW added with EAS and EAS alone even if the reduction extents differed among the types of FVW. Apparent reductions were not found in the bed compartment where the final products accumulated. For the fresh cast, the relative abundances of ARGs and intl 1 against to the total bacterial 16S rDNA decreased markedly. The present study provided an insight for proper controlling of ARGs during vermicomposting of FVW and EAS.
利用新型蚯蚓生物反应器（由基质和床层两部分组成），研究了混合过量活性污泥（EAS）以有效处理不同水果蔬菜废物（FVW）时，抗生素抗性基因（ARGs）的消除情况。选择了 tet G、tet M 和 sul 1 这 3 种 ARGs 以及移动遗传元件基因（intl 1）作为目标基因，并通过定量分析它们在基质和新鲜蚯蚓体内的丰度，证实了添加 EAS 和仅添加 EAS 的 FVW 处理在基质部分的减少，即使 FVW 类型不同，减少程度也有所不同。在最终产物积累的床层部分没有发现明显的减少。对于新鲜的蚯蚓，ARGs 和 intl 1 相对于总细菌 16S rDNA 的相对丰度显著下降。本研究为控制 FVW 和 EAS 蚯蚓堆肥过程中 ARGs 提供了深入的见解。
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