Viegas Carla, Dias Marta, Monteiro Ana, Faria Tiago, Lage Joana, Carolino Elisabete, Caetano Liliana Aranha, Gomes Anita Quintal, Almeida Susana Marta, Verde Sandra Cabo, Belo Joana, Canha Nuno
H&TRC- Health & Technology Research Center, ESTeSL- Escola Superior de Tecnologia da Saúde, Instituto Politécnico de, Lisboa, Portugal; NOVA National School of Public Health, Public Health Research Centre, Universidade NOVA de, Lisboa, Portugal; Comprehensive Health Research Center (CHRC), Portugal.
H&TRC- Health & Technology Research Center, ESTeSL- Escola Superior de Tecnologia da Saúde, Instituto Politécnico de, Lisboa, Portugal.
Environ Pollut. 2021 Jan 4;273:116417. doi: 10.1016/j.envpol.2020.116417.
A wider characterization of indoor air quality during sleep is still lacking in the literature. This study intends to assess bioburden before and after sleeping periods in Portuguese dwellings through active methods (air sampling) coupled with passive methods, such as electrostatic dust cloths (EDC); and investigate associations between before and after sleeping and bioburden. In addition, and driven by the lack of information regarding fungi azole-resistance in Portuguese dwellings, a screening with supplemented media was also performed. The most prevalent genera of airborne bacteria identified in the indoor air of the bedrooms were Micrococcus (41%), Staphylococcus (15%) and Neisseria (9%). The major indoor bacterial species isolated in all ten studied bedrooms were Micrococcus luteus (30%), Staphylococcus aureus (13%) and Micrococcus varians (11%). Our results highlight that our bodies are the source of the majority of the bacteria found in the indoor air of our homes. Regarding air fungal contamination, Chrysosporium spp. presented the highest prevalence both in after the sleeping period (40.8%) and before the sleeping period (28.8%) followed by Penicillium spp. (23.47% morning; 23.6% night) and Chrysonilia spp. (12.4% morning; 20.3% night). Several Aspergillus sections were identified in air and EDC samples. However, none of the fungal species/strains (Aspergillus sections Fumigati, Flavi, Nidulantes and Circumdati) were amplified by qPCR in the analyzed EDC. The correlations observed suggest reduced susceptibility to antifungal drugs of some fungal species found in sleeping environments. Toxigenic fungal species and indicators of harmful fungal contamination were observed in sleeping environments.
目前文献中仍缺乏对睡眠期间室内空气质量更广泛的描述。本研究旨在通过主动方法（空气采样）结合被动方法，如静电除尘布（EDC），评估葡萄牙住宅睡眠前后的生物负荷；并研究睡眠前后与生物负荷之间的关联。此外，由于缺乏葡萄牙住宅中真菌唑抗性的信息，还进行了补充培养基的筛选。在卧室室内空气中鉴定出的最常见的空气传播细菌属是微球菌属（41%）、葡萄球菌属（15%）和奈瑟菌属（9%）。在所有十个研究卧室中分离出的主要室内细菌种类是藤黄微球菌（30%）、金黄色葡萄球菌（13%）和变异微球菌（11%）。我们的结果突出表明，我们的身体是家中室内空气中发现的大多数细菌的来源。关于空气真菌污染，金孢子菌属在睡眠后（40.8%）和睡眠前（28.8%）的患病率最高，其次是青霉属（早上23.47%；晚上23.6%）和金色链霉菌属（早上12.4%；晚上20.3%）。在空气和EDC样本中鉴定出了几个曲霉组。然而，在分析的EDC中，通过qPCR未扩增出任何真菌种类/菌株（曲霉组烟曲霉、黄曲霉、构巢曲霉和环纹曲霉）。观察到的相关性表明，睡眠环境中发现的一些真菌种类对抗真菌药物的敏感性降低。在睡眠环境中观察到了产毒真菌种类和有害真菌污染的指标。
