Hospital Sírio-Libanês, Brasília, DF, Brazil.
Genesis, Centro de Assistência em Reprodução Humana, Brasília, DF, Brazil.
Rev Bras Ginecol Obstet. 2021 Mar;43(3):225-231. doi: 10.1055/s-0040-1718436. Epub 2021 Jan 19.
We report a case of ultrasound-guided ex vivo oocyte retrieval for fertility preservation in a woman with bilateral borderline ovarian tumor, for whom conventional transvaginal oocyte retrieval was deemed unsafe because of the increased risk of malignant cell spillage. Ovarian stimulation with gonadotropins was performed. Surgery was scheduled according to the ovarian response to exogenous gonadotropic stimulation; oophorectomized specimens were obtained by laparoscopy, and oocyte retrieval was performed ∼ 37 hours after the ovulatory trigger. The sum of 20 ovarian follicles were aspirated, and 16 oocytes were obtained. We performed vitrification of 12 metaphase II oocytes and 3 oocytes matured in vitro. Our result emphasizes the viability of ex vivo mature oocyte retrieval after controlled ovarian stimulation for those with high risk of malignant dissemination by conventional approach.
我们报告了一例超声引导下的离体卵母细胞取卵用于生育保存的病例，该患者患有双侧交界性卵巢肿瘤，由于传统的经阴道卵母细胞取卵存在恶性细胞溢出的风险增加，因此被认为是不安全的。对患者进行了促性腺激素的卵巢刺激。手术时间根据对外源性促性腺激素刺激的卵巢反应来安排；通过腹腔镜获取卵巢切除标本，并在排卵诱导后约 37 小时进行卵母细胞取卵。抽吸了 20 个卵巢卵泡，获得了 16 个卵母细胞。我们对 12 个 MII 期卵母细胞和 3 个体外成熟的卵母细胞进行了玻璃化冷冻。我们的结果强调了对于那些通过传统方法恶性扩散风险高的患者，在控制性卵巢刺激后进行离体成熟卵母细胞取卵的可行性。
