Li Shuai, Zhang Hai-Guang, Li Dong-Dong, Wu Jian-Ping, Sun Cheng-Yan, Hu Qing-Xi
Rapid Manufacturing Engineering Center, Shanghai University, Shanghai 200444, China.
Shanghai Key Laboratory of Intelligent Manufacturing and Robotics, Shanghai University, Shanghai 200072, China.
ACS Biomater Sci Eng. 2017 Oct 9;3(10):2493-2501. doi: 10.1021/acsbiomaterials.7b00355. Epub 2017 Sep 1.
Despite significant progress in the fabrication of prevascularized networks over the past decade, a number of challenges remain. One of the most relevant issues is the lack of three-dimensional (3D) structures, which limits the clinical applications of the engineered scaffolds. Another problem is the complexity of prevascularized networks in engineered scaffolds, which is still less than that of human tissues, especially in the case of mature and bulk tissues. Thus, there is still the need to develop more flexible methods to better simulate the structure of natural tissues. In this work, we used a versatile sacrificial template method to fabricate bulk scaffolds with spatial prevascularized networks. Soft poly(vinyl alcohol) (PVA) filaments were used to print the sacrificial template, and the receiving platform was a stepped shaft, allowing the sacrificial template to have a complex 3D structure. The obtained template was embedded into gelatin and microbial transglutaminase (mTG). The inner PVA template could be extracted from the enzymatic cross-linking system, and an engineered scaffold with spatial prevascularized networks was obtained. In vitro experiments demonstrated that the fabrication process is biocompatible with cells.
尽管在过去十年中，预血管化网络的构建取得了显著进展，但仍存在一些挑战。其中最相关的问题之一是缺乏三维（3D）结构，这限制了工程支架的临床应用。另一个问题是工程支架中预血管化网络的复杂性，其仍低于人体组织，尤其是在成熟和大块组织的情况下。因此，仍然需要开发更灵活的方法来更好地模拟天然组织的结构。在这项工作中，我们使用了一种通用的牺牲模板法来制造具有空间预血管化网络的大块支架。柔软的聚乙烯醇（PVA）细丝用于打印牺牲模板，接收平台是一个阶梯轴，使牺牲模板具有复杂的3D结构。将获得的模板嵌入明胶和微生物转谷氨酰胺酶（mTG）中。内部PVA模板可从酶交联系统中提取，从而获得具有空间预血管化网络的工程支架。体外实验表明，该制造过程与细胞具有生物相容性。
