Al Rugaie Osamah, Jabir Majid, Kadhim Rua, Karsh Esraa, Sulaiman Ghassan M, Mohammed Salman A A, Khan Riaz A, Mohammed Hamdoon A
Department of Basic Medical Sciences, College of Medicine and Medical Sciences, Qassim University, Unaizah, P.O. Box 991, Al-Qassim 51911, Saudi Arabia.
Department of Applied Sciences, Division of Biotechnology, University of Technology, Baghdad 35010, Iraq.
Microorganisms. 2021 Jan 5;9(1):101. doi: 10.3390/microorganisms9010101.
Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly ( < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria and . Under experimental conditions, upon bacterial exposure, the combined GNPs and GOFs induced significant clearance of bacteria through phagosome maturation ( < 0.0001) from time-points of 6 to 30 min and production of reactive oxygen species (ROS, < 0.0001) through the NADPH oxidase 2 (NOX2, < 0.0001)-based feedback mechanism. The effects of the combined presence of GNPs and GOFs on phagocytosis ( < 0.0001) suggested a synergistic action underway, also achieved through elevated signal transduction activity in the bone-marrow-derived macrophages (BMDM, < 0.0001). The current study demonstrated that GNPs' and GOFs' bactericidal assisting potentials could be considered an effective and alternative strategy for treating infections from both positive and negative bacterial strains.
金纳米颗粒（GNPs）和氧化石墨烯片（GOFs）对具有吞噬性质的免疫细胞针对革兰氏阳性和革兰氏阴性人类病原菌的吞噬生物活性发挥了显著作用（<0.0001）。在实验条件下，细菌暴露后，从6至30分钟的时间点起，GNPs和GOFs的组合通过吞噬体成熟诱导细菌显著清除（<0.0001），并通过基于烟酰胺腺嘌呤二核苷酸磷酸氧化酶2（NOX2，<0.0001）的反馈机制产生活性氧（ROS，<0.0001）。GNPs和GOFs共同存在对吞噬作用的影响（<0.0001）表明正在发生协同作用，这也通过骨髓来源巨噬细胞（BMDM，<0.0001）中信号转导活性的提高得以实现。当前研究表明，GNPs和GOFs的杀菌辅助潜力可被视为治疗阳性和阴性细菌菌株感染的一种有效且替代的策略。
