Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
Molecules. 2021 Jan 5;26(1):243. doi: 10.3390/molecules26010243.
Continued expansion of the chemical biology toolbox presents many new and diverse opportunities to interrogate the fundamental molecular mechanisms driving complex plant-microbe interactions. This review will examine metabolic labeling with click chemistry reagents and activity-based probes for investigating the impacts of plant-associated microbes on plant growth, metabolism, and immune responses. While the majority of the studies reviewed here used chemical biology approaches to examine the effects of pathogens on plants, chemical biology will also be invaluable in future efforts to investigate mutualistic associations between beneficial microbes and their plant hosts.
化学生物学工具包的持续扩展为研究复杂的植物-微生物相互作用的基本分子机制提供了许多新的和多样化的机会。本文综述了利用点击化学试剂和基于活性的探针进行代谢标记，以研究植物相关微生物对植物生长、代谢和免疫反应的影响。虽然这里回顾的大多数研究都使用了化学生物学方法来研究病原体对植物的影响，但化学生物学在未来研究有益微生物与其植物宿主之间的互利关系方面也将具有重要价值。
