Maione Angela, de Alteriis Elisabetta, Carraturo Federica, Galdiero Stefania, Falanga Annarita, Guida Marco, Di Cosmo Anna, Maselli Valeria, Galdiero Emilia
Department of Biology, University of Naples Federico II, Via Cinthia 26, 80126 Naples, Italy.
Department of Pharmacy, School of Medicine, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy.
J Fungi (Basel). 2021 Jan 5;7(1):26. doi: 10.3390/jof7010026.
The antibiofilm activity of a gH625 analogue was investigated to determine the in vitro inhibition and eradication of a dual-species biofilm of and , two leading opportunistic pathogens responsible for several resistant infections. The possibility of effectively exploiting this peptide as an alternative anti-biofilm strategy in vivo was assessed by the investigation of its efficacy on the larvae model. Results on larvae survival demonstrate a prophylactic efficacy of the peptide towards the infection of each single microorganism but mainly towards the co-infection. The expression of biofilm-related genes in vivo showed a possible synergy in virulence when these two species co-exist in the host, which was effectively prevented by the peptide. These findings provide novel insights into the treatment of medically relevant bacterial-fungal interaction.
研究了gH625类似物的抗生物膜活性，以确定其对两种主要的机会性病原体——[具体病原体1]和[具体病原体2]的双物种生物膜的体外抑制和根除作用，这两种病原体导致了几种耐药性感染。通过研究其在[幼虫模型名称]幼虫模型上的功效，评估了在体内有效利用这种肽作为替代抗生物膜策略的可能性。幼虫存活结果表明，该肽对每种单一微生物的感染具有预防功效，但主要是对共感染的预防功效。体内生物膜相关基因的表达表明，当这两种物种在宿主体内共存时，在毒力方面可能存在协同作用，而该肽有效地阻止了这种协同作用。这些发现为治疗医学上相关的细菌 - 真菌相互作用提供了新的见解。
