Vrancheva Radka, Ivanov Ivan, Dincheva Ivayla, Badjakov Ilian, Pavlov Atanas
Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria.
Department of Organic Chemistry and Inorganic Chemistry, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria.
Plants (Basel). 2021 Jan 5;10(1):94. doi: 10.3390/plants10010094.
The purpose of the current study was to identify and quantify triterpenoids and other non-polar compounds in the leaves of three high bush blueberry cultivars ( L. var. Bluegold, var. Bluecrop and var. Elliott) and three natural populations of species ( L., L. and L.) by means of gas chromatography mass spectrometry (GC-MS) and high-performance liquid chromatography with diode array detector (HPLC-DAD). Metabolite profiles differed significantly among the species analyzed, as well as among the populations of the same species. The populations of predominantly contained relative concentrations of phytosterols (varying between 10.48% of total ion current (TIC) and 22.29% of TIC) and almost twice the content of triterpenes (from 29.84% of TIC to 49.62% of TIC) of the other berry species investigated. The leaves of varieties biosynthesized the highest relative amount of fatty acids, while the leaves of the populations of had the highest relative concentrations of fatty alcohols. The results of principal component analysis (PCA) and hierarchical cluster analysis (HCA) showed that the diverse populations of each berry species analyzed differed from each other, most likely due to variations in the climatic and geographical conditions of their localities.
本研究的目的是通过气相色谱 - 质谱联用（GC - MS）和带二极管阵列检测器的高效液相色谱法（HPLC - DAD），对三个高丛蓝莓品种（蓝金蓝莓、蓝丰蓝莓和艾略特蓝莓）的叶片以及三种野生蓝莓（笃斯越桔、红豆越桔和欧洲越桔）的三个自然种群中的三萜类化合物和其他非极性化合物进行鉴定和定量。在所分析的不同蓝莓种类之间以及同一种类的不同种群之间，代谢物谱存在显著差异。笃斯越桔种群主要含有相对浓度的植物甾醇（占总离子流（TIC）的10.48%至22.29%），其三萜类化合物含量几乎是其他所研究浆果种类的两倍（从占TIC的29.84%至49.62%）。蓝金蓝莓品种的叶片生物合成的脂肪酸相对量最高，而红豆越桔种群的叶片中脂肪醇的相对浓度最高。主成分分析（PCA）和层次聚类分析（HCA）结果表明，所分析的每个浆果种类的不同种群彼此不同，这很可能是由于其所在地区气候和地理条件的差异所致。
