Institute of Chemical Process Fundamentals of the CAS, v. v. i., Rozvojová 135/1, 16502 Prague, Czech Republic.
Faculty of Environment, Jan Evangelista Purkyně University in Ústí nad Labem, Pasteurova 3632/15, 40096 Ústí nad Labem, Czech Republic.
Sensors (Basel). 2021 Jan 14;21(2):565. doi: 10.3390/s21020565.
Glucose belongs among the most important substances in both physiology and industry. Current food and biotechnology praxis emphasizes its on-line continuous monitoring and regulation. These provoke increasing demand for systems, which enable fast detection and regulation of deviations from desired glucose concentration. We demonstrated control of glucose concentration by feedback regulation equipped with in situ optical fiber glucose sensor. The sensitive layer of the sensor comprises oxygen-dependent ruthenium complex and preimmobilized glucose oxidase both entrapped in organic-inorganic polymer ORMOCER. The sensor was placed in the laboratory bioreactor (volume 5 L) to demonstrate both regulations: the control of low levels of glucose concentrations (0.4 and 0.1 mM) and maintenance of the glucose concentration (between 2 and 3.5 mM) during stationary phase of cultivation of . Response times did not exceed 6 min (average 4 min) with average deviation of 4%. Due to these regulation characteristics together with durable and long-lasting (≥2 month) sensitive layer, this feedback regulation system might find applications in various biotechnological processes such as production of low glucose content beverages.
葡萄糖在生理和工业中都是最重要的物质之一。当前的食品和生物技术实践强调对其进行在线连续监测和调节。这些因素促使人们对能够快速检测和调节偏离所需葡萄糖浓度的系统的需求不断增加。我们通过配备原位光纤葡萄糖传感器的反馈调节来控制葡萄糖浓度。传感器的敏感层由氧依赖性钌配合物和预先固定在有机-无机聚合物 ORMOCER 中的葡萄糖氧化酶组成。该传感器被放置在实验室生物反应器（5 升体积）中，以演示两种调节：低水平葡萄糖浓度（0.4 和 0.1mM）的控制，以及培养静止阶段葡萄糖浓度（2 至 3.5mM 之间）的维持。响应时间不超过 6 分钟（平均 4 分钟），平均偏差为 4%。由于这些调节特性以及耐用且持久（≥2 个月）的敏感层，这种反馈调节系统可能在各种生物技术过程中找到应用，例如生产低葡萄糖含量的饮料。
