ENNOVA Institute of Life Science and Technology, ENN Group, South District of ENN Industrial Park, Langfang, 065001, Hebei, China.
Sci Rep. 2022 Jan 10;12(1):394. doi: 10.1038/s41598-021-04290-9.
Delayed luminescence (DL) is gradually used in various detection of biological systems as a rapid detection technique, however, its biological mechanism was still not clear. In this study, a new model of DL detection system for liquid biological samples is established to investigate the DL emission of Saccharomyces cerevisiae cells cultured in different glucose concentrations. We analyzed the relationship between the DL emission and cell growth, cell vitality, mitochondrial morphology, mitochondrial DNA (mtDNA) copy number, adenosine triphosphate (ATP), oxygen consumption rate (OCR), as well as mitochondria membrane potential (MMP) in S. cerevisiae cells cultured with 0.01, 0.05, 0.15, 3, 10 and 20 g/L glucose respectively. It was found that the DL emission had strong correlation with mitochondrial morphology, OCR, and MMP. The results suggested that DL is an indicator of mitochondria status under different glucose supply conditions, and may be an effective method to detect mitochondrial metabolism related disorders.
延迟发光（DL）逐渐被用作一种快速检测技术，用于各种生物系统的检测，但其生物学机制尚不清楚。本研究建立了一种新的液体生物样品 DL 检测系统模型，以研究不同葡萄糖浓度下培养的酿酒酵母细胞的 DL 发射。我们分析了 DL 发射与细胞生长、细胞活力、线粒体形态、线粒体 DNA（mtDNA）拷贝数、三磷酸腺苷（ATP）、耗氧量（OCR）以及在 0.01、0.05、0.15、3、10 和 20 g/L 葡萄糖下培养的酿酒酵母细胞的线粒体膜电位（MMP）之间的关系。结果表明，DL 发射与线粒体形态、OCR 和 MMP 具有很强的相关性。结果表明，DL 是不同葡萄糖供应条件下线粒体状态的一个指标，可能是检测与线粒体代谢相关的疾病的有效方法。
