Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China.
Asian Natural Environmental Science Center (ASNESC), The University of Tokyo, Nishitokyo, Tokyo 188-0002, Japan.
Int J Mol Sci. 2021 Jan 16;22(2):856. doi: 10.3390/ijms22020856.
High-capacity tonoplast cation/H antiport in plants is partially mediated by a family of CAX transporters. Previous studies have reported that CAX activity is affected by an N-terminal autoinhibitory region. CAXs may be present as heterodimers in plant cells, and this phenomenon necessitates further study. In this study, we demonstrate that there is an interaction between CAX4 and CAX1 as determined by the use of a yeast two-hybrid system and a bimolecular fluorescence complementation assay. More specifically, the N-terminal of CAX4 interacts with CAX1. We further observed the over-expression and either a single or double mutant of and in response to abiotic stress in . These results suggest that CAX1 and CAX4 can interact to form a heterodimer, and the N-terminal regions of CAX4 play important roles in vivo; this may provide a foundation for a deep study of CAX4 function in the future.
植物中的高容量液泡阳离子/H 反向转运蛋白部分由 CAX 转运蛋白家族介导。先前的研究报告称，CAX 活性受 N 端自动抑制区域的影响。CAX 可能在植物细胞中以异源二聚体的形式存在，这一现象需要进一步研究。在这项研究中，我们通过酵母双杂交系统和双分子荧光互补测定证实了 CAX4 和 CAX1 之间存在相互作用。更具体地说，CAX4 的 N 端与 CAX1 相互作用。我们还观察到在非生物胁迫下，CAX1 和 CAX4 在 中过量表达，并且要么是单个或双突变体。这些结果表明 CAX1 和 CAX4 可以相互作用形成异源二聚体，并且 CAX4 的 N 端区域在体内发挥重要作用；这可能为未来深入研究 CAX4 功能提供基础。
