Nekrasov Nikita, Yakunina Natalya, Pushkarev Averyan V, Orlov Alexey V, Gadjanski Ivana, Pesquera Amaia, Centeno Alba, Zurutuza Amaia, Nikitin Petr I, Bobrinetskiy Ivan
National Research University of Electronic Technology, 124498 Moscow, Russia.
Moscow Institute of Physics and Technology, 9 Institutskii per., Dolgoprudny, 141700 Moscow, Russia.
Nanomaterials (Basel). 2021 Jan 16;11(1):226. doi: 10.3390/nano11010226.
In this work, we report a novel method of label-free detection of small molecules based on direct observation of interferometric signal change in graphene-modified glasses. The interferometric sensor chips are fabricated via a conventional wet transfer method of CVD-grown graphene onto the glass coverslips, lowering the device cost and allowing for upscaling the sensor fabrication. For the first time, we report the use of graphene functionalized by the aptamer as the bioreceptor, in conjunction with Spectral-Phase Interferometry (SPI) for detection of ochratoxin A (OTA). In a direct assay with an OTA-specific aptamer, we demonstrated a quick and significant change of the optical signal in response to the maximum tolerable level of OTA concentration. The sensor regeneration is possible in urea solution. The developed platform enables a direct method of kinetic analysis of small molecules using a low-cost optical chip with a graphene-aptamer sensing layer.
在本工作中，我们报告了一种基于直接观察石墨烯修饰玻璃中干涉信号变化的小分子无标记检测新方法。干涉传感器芯片通过将化学气相沉积（CVD）生长的石墨烯以传统湿法转移到玻璃盖玻片上制成，降低了器件成本并有利于扩大传感器制造规模。我们首次报告了使用经适配体功能化的石墨烯作为生物受体，结合光谱相位干涉术（SPI）来检测赭曲霉毒素A（OTA）。在与OTA特异性适配体的直接检测中，我们证明了响应OTA浓度的最大耐受水平时光学信号会快速且显著地变化。该传感器在尿素溶液中可实现再生。所开发的平台利用具有石墨烯 - 适配体传感层的低成本光学芯片，实现了小分子动力学分析的直接方法。
