State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricutural University, Nanjing 210095, China.
Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture, College of Horticulture of Nanjing Agricultural University, Nanjing 210095, China.
Genes (Basel). 2021 Jan 15;12(1):102. doi: 10.3390/genes12010102.
The LBD (Lateral Organ Boundaries Domain) family are a new group of plant-specific genes, which encode a class of transcription factors containing conserved Lateral Organization Boundary (LOB) domains, and play an important role in regulating the adaxial-abaxial polarity of plant leaves. In , ASYMMETRIC LEAVES 2 () has a typical LOB domain and is involved in determining the adaxial cell fate. In this study, we isolated the gene from the pak choi cultivar "NHCC001", and analyzed its expression pattern. The results showed that the encoded a protein made up of 202 amino acid residues which were located in the nucleus and cytomembrane. The Yeast two-hybrid system (Y2H) assay indicated that interacts with and (the homologous genes of gene in pak choi). In the transgenic that overexpressed gene, it presented an abnormal phenotype with a curly shape. Taken together, our findings not only validate the function of in leaf development in , but also contribute in unravelling the molecular regulatory mechanism of , which fulfills a special role by forming complexes with in the establishment of the adaxial-abaxial polarity of the lateral organs in pak choi.
LBD（侧生器官边界域）家族是一组新的植物特异性基因，它们编码一类转录因子，包含保守的侧生组织边界（LOB）结构域，在调节植物叶片的近轴-远轴极性方面发挥重要作用。在 中，ASYMMETRIC LEAVES 2()具有典型的 LOB 结构域，参与确定近轴细胞命运。在本研究中，我们从白菜品种“NHCC001”中分离出 基因，并分析其表达模式。结果表明，该基因编码的蛋白由 202 个氨基酸残基组成，定位于细胞核和细胞质膜。酵母双杂交系统（Y2H）试验表明，与 和 （白菜 基因的同源基因）相互作用。在过表达 基因的转基因 中，表现出卷曲的异常表型。总之，我们的研究结果不仅验证了 基因在 中叶片发育中的功能，而且还揭示了 基因的分子调控机制，通过与 形成复合物，在白菜侧生器官的近轴-远轴极性的建立中发挥特殊作用。
