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通过直接激活转录调控不结球白菜叶片近轴极性发育

Regulates Leaf Adaxial Polarity Development in Non-Heading Chinese Cabbage by Directly Activating Transcription.

作者信息

Jiang Cheng, Ding Qiang, He Ying, Li Yiran, Gao Zhanyuan, Li Entong, Hou Xilin

机构信息

National Key Laboratory of Crop Genetics & Germplasm Innovation and Utilization, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, Nanjing 210095, China.

Nanjing Suman Plasma Engineering Research Institute Co., Ltd., Nanjing 211162, China.

出版信息

Plants (Basel). 2025 Apr 14;14(8):1207. doi: 10.3390/plants14081207.

DOI:10.3390/plants14081207
PMID:40284095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12030544/
Abstract

Leaves are the primary organs for plant photosynthesis, and their flat, symmetric morphology is crucial for plant growth and development. The LBD family transcription factor () plays a central role in the establishment of leaf polarity. In this study, we cloned the gene from the non-heading Chinese cabbage cultivar "NHCC001" and successfully generated overexpression strains through genetic transformation. Phenotypic analysis revealed that overexpression of led to significant upward curling of leaves in non-heading Chinese cabbage. Additionally, we found that the expression of , a gene associated with leaf adaxial polarity development, was significantly up-regulated in BcAS2-overexpressing plants compared to controls. This interaction was further confirmed through yeast one-hybridization (Y1H), dual-luciferase reporter assays, and electrophoretic mobility shift assay (EMSA), all of which demonstrated that directly binds to the GATA-motif site of the promoter and promotes its transcription. Functional validation via overexpression and silencing of confirmed its role in regulating adaxial polarity development in non-heading Chinese cabbage leaves. This study elucidates the molecular mechanism of the BcAS2-BcPHB pathway in regulating leaf polarity in non-heading Chinese cabbage, providing a theoretical foundation for morphological improvement breeding.

摘要

叶片是植物光合作用的主要器官，其扁平、对称的形态对植物的生长发育至关重要。LBD家族转录因子（）在叶片极性的建立中起核心作用。在本研究中，我们从不结球白菜品种“NHCC001”中克隆了基因，并通过遗传转化成功构建了过表达株系。表型分析表明，过表达导致不结球白菜叶片显著向上卷曲。此外，我们发现，与叶片近轴极性发育相关的基因在BcAS2过表达植株中的表达相较于对照显著上调。通过酵母单杂交（Y1H）、双荧光素酶报告基因检测和电泳迁移率变动分析（EMSA）进一步证实了这种相互作用，所有这些都表明直接结合到启动子的GATA基序位点并促进其转录。通过过表达和沉默进行功能验证，证实了其在调控不结球白菜叶片近轴极性发育中的作用。本研究阐明了BcAS2 - BcPHB途径调控不结球白菜叶片极性的分子机制，为形态改良育种提供了理论基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/5e4366818628/plants-14-01207-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/bc9e53675691/plants-14-01207-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/3e8bb119c999/plants-14-01207-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/86266197bcce/plants-14-01207-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/093c41fac6dc/plants-14-01207-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/eea47eedb837/plants-14-01207-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/a4adb2ad9db1/plants-14-01207-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/06e80325ae20/plants-14-01207-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/5e4366818628/plants-14-01207-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/bc9e53675691/plants-14-01207-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/3e8bb119c999/plants-14-01207-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/86266197bcce/plants-14-01207-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/093c41fac6dc/plants-14-01207-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/eea47eedb837/plants-14-01207-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/a4adb2ad9db1/plants-14-01207-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/06e80325ae20/plants-14-01207-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6361/12030544/5e4366818628/plants-14-01207-g008.jpg

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本文引用的文献

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Regulatory interaction of BcWRKY33A and BcHSFA4A promotes salt tolerance in non-heading Chinese cabbage [ (syn. ) ssp. ].BcWRKY33A与BcHSFA4A的调控相互作用促进了不结球白菜（[同义词]亚种）的耐盐性。
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CELL CYCLE SEITCH 52 regulates tillering by interacting with LATERAL SUPPRESSOR in non-heading Chinese cabbage.
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