El-Kaliuoby Mai I, Khalil Alaa M, El-Khatib Ahmed M, Shehata Nader
Faculty of Education, Alexandria University, Alexandria 21544, Egypt.
Faculty of Engineering, Pharos University in Alexandria, Alexandria 21544, Egypt.
Polymers (Basel). 2021 Jan 15;13(2):277. doi: 10.3390/polym13020277.
The over-reliance on antibiotics and their enormous misuse has led to warnings of a future without effective medicines and so, the need for alternatives to antibiotics has become a must. Non-traditional antibacterial treatment was performed by using an aray of nanocomposites synergised with exposure to electromagnetic waves. In this manuscript, electrospun poly(vinyl alcohol) (PVA) nanofiber mats embedded with silver nanoparticles (Ag NPs) were synthesized. The nanocomposites were characterized by Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM), Current-Voltage (I-V) curves, and Thermogravimetric analysis (TGA) along with analysis of antibacterial impact against and bacteria, studied by bacterial growing analysis, growth kinetics, and cellular cytotoxicity. The results indicated a spherical grain shape of silver of average size 20 nm and nanofibers' mean diameter of less than 100 nm. The nanocomposite mats showed good exposure to bacteria and the ability to sustain release of silver for a relatively long time. Moreover, the applied electromagnetic waves (EMWs) were shown to be a synergistic co-factor in killing bacteria even at low concentrations of Ag NPs. This caused pronounced alterations of the bacterial preserved packing of the cell membrane. Thereby, the treatment with nanocomposite mats under EM wave exposure elucidated maximum inhibition for both bacterial strains. It was concluded that the functioning of nanofiber with silver nanoparticles and exposure to electromagnetic waves improved the antibacterial impact compared to each one alone.
对抗生素的过度依赖及其大量滥用已引发了对未来无有效药物的警告，因此，寻找抗生素替代品已成为必然需求。采用一系列与电磁波协同作用的纳米复合材料进行非传统抗菌治疗。在本论文中，合成了嵌入银纳米颗粒（Ag NPs）的静电纺聚（乙烯醇）（PVA）纳米纤维垫。通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）、电流-电压（I-V）曲线和热重分析（TGA）对纳米复合材料进行了表征，并通过细菌生长分析、生长动力学和细胞毒性研究了其对两种细菌的抗菌作用。结果表明，银的球形颗粒平均尺寸为20 nm，纳米纤维的平均直径小于100 nm。纳米复合垫对细菌具有良好的接触性，并能在较长时间内持续释放银。此外，即使在低浓度的Ag NPs下，所施加的电磁波（EMWs）也被证明是杀灭细菌的协同辅助因素。这导致细菌细胞膜的保留包装发生明显改变。因此，在电磁波照射下用纳米复合垫进行治疗对两种细菌菌株均显示出最大抑制作用。得出的结论是，与单独使用纳米纤维或银纳米颗粒相比，含银纳米颗粒的纳米纤维与电磁波照射共同作用可增强抗菌效果。
