El-Kaliuoby Mai I, Amer Motaz, Shehata Nader
Faculty of Education, Alexandria University, Alexandria 21544, Egypt.
Basic and Applied Science Institute, College of Engineering Arab Academy for Science, Technology and Maritime Transports, Alexandria 21544, Egypt.
Polymers (Basel). 2021 Jun 4;13(11):1869. doi: 10.3390/polym13111869.
Chronic wounds are commonly colonized with bacteria in a way that prevents full healing process and capacity for repair. Nano-chitosan, a biodegradable and nontoxic biopolymer, has shown bacteriostatic activity against a wide spectrum of bacteria. Effectively, pulsed electromagnetic fields are shown to have both wound healing enhancement and antibacterial activity. This work aimed to combine the use of nano-chitosan and exposure to a pulsed electric field to overcome two common types of infectious bacteria, namely and . Here, bacteria growing rate, growth kinetics and cell cytotoxicity (levels of lactate dehydrogenase, protein leakage and nucleic acid leakage) were investigated. Our findings confirmed the maximum antibacterial synergistic combination of nano-chitosan and exposure against than using each one alone. It is presumed that the exposure has influenced bacteria membrane charge distribution in a manner that allowed more chitosan to anchor the surface and enter inside the cell. Significantly, cell cytotoxicity substantiates high enzymatic levels as a result of cell membrane disintegration. In conclusion, exposure to pulsed electromagnetic fields has a synergistic antibacterial effect against and with maximum inhibitory effect for the last one. Extensive work should be done to evaluate the combination against different bacteria types to get general conclusive results. The ability of using pulsed electromagnetic fields as a wound healing accelerator and antibacterial cofactor has been proved, but in vivo experimental work in the future to verify the use of such a new combination against infectious wounds and to determine optimum treatment conditions is a must.
慢性伤口通常会被细菌定植，从而阻碍伤口的完全愈合过程和修复能力。纳米壳聚糖是一种可生物降解且无毒的生物聚合物，已显示出对多种细菌具有抑菌活性。有效地，脉冲电磁场已显示出具有促进伤口愈合和抗菌活性。这项工作旨在结合使用纳米壳聚糖和暴露于脉冲电场来克服两种常见的感染性细菌，即 和 。在此，研究了细菌生长速率、生长动力学和细胞毒性（乳酸脱氢酶水平、蛋白质泄漏和核酸泄漏）。我们的研究结果证实，与单独使用纳米壳聚糖或暴露于脉冲电场相比，纳米壳聚糖与暴露于脉冲电场的组合具有最大的抗菌协同作用。据推测，暴露以一种允许更多壳聚糖锚定在表面并进入细胞内部的方式影响了细菌膜电荷分布。值得注意的是，由于细胞膜解体，细胞毒性证实了高酶水平。总之，暴露于脉冲电磁场对 和 具有协同抗菌作用，对后者具有最大抑制作用。应该进行大量工作来评估针对不同细菌类型的组合，以获得一般性的结论性结果。使用脉冲电磁场作为伤口愈合促进剂和抗菌辅助因子的能力已得到证明，但未来必须进行体内实验工作，以验证这种新组合对感染性伤口的使用，并确定最佳治疗条件。
