Characterization and Function of the 1-Deoxy-D-xylose-5-Phosphate Synthase (DXS) Gene Related to Terpenoid Synthesis in .

In the methyl-D-erythritol-4-phosphate (MEP) pathway, 1-deoxy-D-xylose-5-phosphate synthase (DXS) is considered the key enzyme for the biosynthesis of terpenoids. In this study, (MK970590) was isolated from . Bioinformatics analysis revealed homology of MK970590 with DXS proteins from other species. Relative expression analysis suggested that expression was higher in roots than in other plant parts, and the treatment of seedlings with mechanical injury via 15% polyethylene glycol 6000, 10 mM HO, 50 μM ethephon (ETH), 10 mM methyl jasmonate (MeJA), and 1 mM salicylic acid (SA) resulted in an increased expression of . pET28a- was expressed in TransB (DE3) cells, and stress analysis showed that the recombinant protein was involved in resistance to NaCl and drought stresses. The subcellular localization of was in the chloroplast. We also cloned a full-length 1024 bp promoter. expression was observed in roots, stems, and leaves. overexpression significantly increased carotenoid, chlorophyll a, and chlorophyll b contents and DXS enzyme activity, suggesting that is important in isoprenoid biosynthesis. This study provides a theoretical basis for molecular breeding for terpene synthesis regulation and resistance.