Zhao Hanxu, Su Jiameng, Zhong Zhaoxuan, Xiong Tongyou, Dai Weicong, Zhang Dongrui, Chang Ying
College of Life Sciences, Northeast Agricultural University, Harbin 150030, China.
Plants (Basel). 2024 Sep 21;13(18):2647. doi: 10.3390/plants13182647.
(L.) Schott has anti-inflammatory and antioxidant properties, and terpenoids are important components of its active constituents. The methyl-D-erythritol 4-phosphate (MEP) pathway is one of the major pathways for the synthesis of terpene precursors in plants, and 1-deoxy-D-xylulose-5-phosphate synthase (DXS) is the first rate-limiting enzyme in this pathway. has been shown to be associated with increased stress tolerance in plants. In this experiment, two genes were extracted from the transcriptome and named and . Based on phylogenetic tree and conserved motif analyses, DXS was shown to be highly conserved evolutionarily and its localization to chloroplasts was determined by subcellular localization. Prokaryotic expression results showed that the number and growth status of recombinant colonies were better than the control under 400 mM NaCl salt stress and 800 mM mannitol-simulated drought stress. In addition, the and transgenic tobacco plants showed improved resistance to drought and salt stress. and responded strongly to methyl jasmonate (MeJA) and PEG-mimicked drought stress following exogenous hormone and abiotic stress treatments of . The transcriptional active sites were investigated by dual luciferase and GUS staining assays, and the results showed that the STRE element (AGGGG), the ABRE element (ACGTGGC), and the MYC element (CATTTG) were the important transcriptional active sites in the promoters of the two genes, which were closely associated with hormone response and abiotic stress. These results suggest that the gene of plays an important role in hormone signaling and response to stress. This study provides a reference for analyzing the molecular mechanisms of stress tolerance in .
(L.) 肖特具有抗炎和抗氧化特性，萜类化合物是其活性成分的重要组成部分。甲基-D-赤藓糖醇4-磷酸（MEP）途径是植物中萜烯前体合成的主要途径之一，1-脱氧-D-木酮糖-5-磷酸合酶（DXS）是该途径中的首个限速酶。已证明其与植物抗逆性增强有关。在本实验中，从转录组中提取了两个基因并命名为 和 。基于系统发育树和保守基序分析，DXS在进化上高度保守，通过亚细胞定位确定其定位于叶绿体。原核表达结果表明，在400 mM NaCl盐胁迫和800 mM甘露醇模拟干旱胁迫下，重组菌落的数量和生长状态优于对照。此外， 和 转基因烟草植株对干旱和盐胁迫的抗性增强。对 进行外源激素和非生物胁迫处理后， 和 对茉莉酸甲酯（MeJA）和聚乙二醇模拟干旱胁迫反应强烈。通过双荧光素酶和GUS染色分析研究转录活性位点，结果表明STRE元件（AGGGG）、ABRE元件（ACGTGGC）和MYC元件（CATTTG）是两个 基因启动子中的重要转录活性位点，它们与激素反应和非生物胁迫密切相关。这些结果表明 的 基因在激素信号传导和胁迫反应中起重要作用。本研究为分析 的抗逆分子机制提供了参考。
