Nuakea Therapeutics, Quincy, Massachusetts, USA
J Bacteriol. 2021 Mar 8;203(7). doi: 10.1128/JB.00698-20.
Transposon insertion sequencing is a useful tool to identify the genes that are essential for a bacterial species to grow and divide effectively. In this issue of , Fabian et al. present the first set of transposon insertion sequencing data highlighting the genes essential to the plant-commensal species strain Pf-5 and describe comparative analyses with other pseudomonads (B. K. Fabian, C. Foster, A. J. Asher, L. D. Elbourne, et al., J Bacteriol 203:e00432-20, 2021, https://doi.org/10.1128/JB.00432-20).
转座子插入测序是一种有用的工具,可以识别对细菌有效生长和分裂至关重要的基因。在本期的 中,Fabian 等人介绍了第一组转座子插入测序数据,这些数据突出了植物共生种 Pf-5 菌株必不可少的基因,并与其他假单胞菌进行了比较分析(B. K. Fabian、C. Foster、A. J. Asher、L. D. Elbourne 等人,J Bacteriol 203:e00432-20,2021,https://doi.org/10.1128/JB.00432-20)。