Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.
J Bacteriol. 2019 Jun 21;201(14). doi: 10.1128/JB.00089-19. Print 2019 Jul 15.
genetics has rapidly advanced in recent years thanks to the development of tools for allelic replacement and transposon mutagenesis. In this issue, Müh extend the genetics toolbox by developing a CRISPRi strategy for gene silencing in (U.Müh, A. G. Pannullo, D. S. Weiss, and C. D. Ellermeier, 2019, J Bacteriol 201:e00711-18. . https://doi.org/10.1128/JB.00711-18). The authors demonstrate the tunability and robustness of their CRISPRi system, highlight its utility in studying essential gene function, and discuss exciting new possibilities for dissecting physiology.
遗传学近年来发展迅速，这要归功于等位基因替换和转座子诱变工具的发展。在本期中，Müh 通过开发用于（U.Müh，A.G.Pannullo，D.S.Weiss 和 C.D.Ellermeier，2019，J Bacteriol 201：e00711-18.. https：//doi.org/10.1128/JB.00711-18）基因沉默的 CRISPRi 策略扩展了遗传学工具箱。作者证明了他们的 CRISPRi 系统的可调性和稳健性，强调了其在研究必需基因功能方面的实用性，并讨论了剖析生理学的令人兴奋的新可能性。
