利用辐射介导的诱变技术证明肺炎克雷伯菌产生碳青霉烯类耐药性的三个必要先决条件。
Proof of the triple prerequisite conditions which are essential for carbapenem resistance development in Klebsiella pneumoniae by using radiation-mediated mutagenesis.
机构信息
Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 03722, South South Korea.
Brain Korea 21+ Project for Medical Science, Yonsei University, Seoul 03722, South Korea.
出版信息
FEMS Microbiol Lett. 2021 Jan 26;368(1). doi: 10.1093/femsle/fnaa189.
Evolution of multi-drug resistant bacteria has led to worldwide research to better understand the various resistance mechanisms in these strains. Every year, novel information on carbapenem resistance and its mechanisms is being discovered. In this study, radiation-mediated mutagenesis was used to transform a carbapenem-resistant Klebsiella pneumoniae strain to a carbapenem-susceptible bacterium. Through this process, we proved three conditions of loss of the OmpK35 and the OmpK36 genes and acquisition of blaCMY-10 worked together to produce carbapenem resistance in K. pneumoniae. Loss of only one of the porins did not evoke carbapenem resistance. This is the first report on the essential contribution of these three components of carbapenem resistance in K. pneumoniae.
多药耐药菌的进化导致了全球范围内的研究，以更好地了解这些菌株中的各种耐药机制。每年都有关于碳青霉烯类耐药性及其机制的新信息被发现。在这项研究中，辐射介导的诱变被用于将耐碳青霉烯类的肺炎克雷伯菌转化为对碳青霉烯类敏感的细菌。通过这个过程，我们证明了 OmpK35 和 OmpK36 基因缺失的三个条件，以及 blaCMY-10 的获得，共同导致了肺炎克雷伯菌对碳青霉烯类的耐药性。只有一种孔蛋白的缺失不会引起碳青霉烯类耐药性。这是关于肺炎克雷伯菌碳青霉烯类耐药性的这三个组成部分的重要贡献的首次报告。
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