Sharma Saloni, Setia Hema, Toor Amrit Pal
University Institute of Engineering and Technology, Panjab University, Chandigarh, India.
Dr. SSB University Institute of Chemical Engineering and Technology, Panjab University, Chandigarh, India.
World J Microbiol Biotechnol. 2021 Jan 20;37(2):33. doi: 10.1007/s11274-021-02998-4.
Among the various pharmaceutical pollutants, diclofenac sodium (DFS), a widely prescribed non-steroid anti-inflammatory drug is detected in the aquatic environment at concentrations which can be harmful to living organisms. Present study illustrates the isolation and characterization of strain Klebsiella pneumoniae WAH1 from activated sludge and its ability to degrade DFS as sole source of carbon and energy. The growth and degradation capacity of K. pneumoniae WAH1 under different conditions of pH, temperature, rotation speed, and inoculum age were evaluated using optical density and liquid chromatography-mass spectroscopy (LCMS). The results show that K. pneumoniae WAH1 can grow well with DFS as its sole source of carbon and degrade 79.14% of DFS (10 mg L) within 72 h. Based on chemical structure of intermediates detected through LCMS, it is inferred that degradation pathway advanced by hydroxylation, decarboxylation, and dechlorination reactions. Toxicity studies revealed the non-toxic nature of the end-products of DFS degradation after 72 h. The findings suggest that K. pneumoniae WAH1 has an excellent potential for bioremediation of DFS in industrial wastewaters.
在各种药物污染物中，双氯芬酸钠（DFS）是一种广泛使用的非甾体抗炎药，在水环境中被检测到的浓度可能对生物有害。本研究阐述了从活性污泥中分离并鉴定肺炎克雷伯菌WAH1菌株，以及该菌株将DFS作为唯一碳源和能源进行降解的能力。利用光密度和液相色谱 - 质谱联用仪（LCMS）评估了肺炎克雷伯菌WAH1在不同pH值、温度、转速和接种龄条件下的生长和降解能力。结果表明，肺炎克雷伯菌WAH1能够以DFS作为唯一碳源良好生长，并在72小时内降解79.14%的DFS（10 mg/L）。基于通过LCMS检测到的中间产物的化学结构，推断其降解途径是通过羟基化、脱羧和脱氯反应进行的。毒性研究表明，72小时后DFS降解终产物无毒性。研究结果表明，肺炎克雷伯菌WAH1在工业废水中对DFS进行生物修复具有巨大潜力。
