Arun Supatcharee, Chaiyamoon Arada, Lapyuneyong Natthapol, Bunsueb Sudtida, Wu Alexander Tsang-Hsien, Iamsaard Sitthichai
Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Research Institute for Human High Performance and Health Promotion (HHP & HP), Khon Kaen University, Khon Kaen, Thailand.
Andrologia. 2021 Apr;53(3):e13981. doi: 10.1111/and.13981. Epub 2021 Jan 20.
Chronic stress (CS) is shown to decrease the semen quality with changed expression of tyrosine phosphorylated (TyrPho) proteins in testicular and seminal tissues. However, the alterations of such proteins and fluid contents in the epididymis, producing sperm maturation factors, have never been reported. Sixteen adult rats were randomly divided into 2 groups (n = 8). The control animals were not subjected to stressors whereas CS rats were immobilised within restraint cage (4 hr/day) before cold forced-water swimming (15 min/day) for 60 days. Corticosterone, testosterone, blood glucose level (BGL), malondialdehyde (MDA) and biochemical components in epididymal fluid were assayed. Expressions of heat shock protein 70 (HSP-70), androgen receptor (AR) and TyrPho protein were investigated in epididymal tissue and fluid. Significantly, CS increased the corticosterone and BGL but decreased testosterone and epididymal substance levels. MDA level in tail epididymal fluid and HSP-70 expression in both regions of epididymal tissues and fluids, except in head epididymal fluid of CS were increased. Epididymal tissues showed the decrease of AR expression. Presence and changes of many TyrPho proteins were observed in CS. In conclusion, CS could affect functional proteins particularly TyrPho in epididymis, resulted in low semen quality.
慢性应激（CS）已被证明会降低精液质量，同时睾丸和精液组织中酪氨酸磷酸化（TyrPho）蛋白的表达也会发生变化。然而，从未有过关于产生精子成熟因子的附睾中此类蛋白质和液体成分变化的报道。将16只成年大鼠随机分为2组（每组n = 8）。对照动物不施加应激源，而CS组大鼠在冷强迫水游泳（每天15分钟）前被固定在束缚笼中（每天4小时），持续60天。检测了皮质酮、睾酮、血糖水平（BGL）、丙二醛（MDA）以及附睾液中的生化成分。研究了附睾组织和液体中热休克蛋白70（HSP - 70）、雄激素受体（AR）和TyrPho蛋白的表达。值得注意的是，CS会使皮质酮和BGL升高，但使睾酮和附睾物质水平降低。除CS组附睾头部液体外，附睾尾部液体中的MDA水平以及附睾组织和液体两个区域中的HSP - 70表达均升高。附睾组织中AR表达降低。在CS组中观察到许多TyrPho蛋白的存在和变化。总之，CS会影响附睾中的功能蛋白，尤其是TyrPho，导致精液质量低下。
