Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei, Taiwan.
Pharm Biol. 2022 Dec;60(1):1935-1943. doi: 10.1080/13880209.2022.2124279.
Although (Wall. ex DC.) Seem (Bignoniaceae) flower (DSF) improves hyperglycaemia, testicular damage and sperm quality in type 2 diabetes mellitus (T2DM) animals, its effects on the seminal vesicles, secreting seminal plasma, are unknown.
This study reports the protective effects of DSF on seminal dysfunction in T2DM rats.
Male Sprague-Dawley rats were divided into four groups (control, T2DM, T2DM + DSF200 and T2DM + DSF600; 10 animals/group). The control group was fed a low-fat diet for 14 days prior to single saline injection, whereas T2DM group was given a high-fat diet and injected with streptozocin (50 mg/kg body weight). The T2DM-induced rats were fed DSF orogastrically (200 and 600 mg/kg body weight) for 28 consecutive days. At the end of the experiment, biochemical components, malondialdehyde (MDA), histology and protein expression in seminal lysate were evaluated.
DSF increased the levels of serum phosphorus (13.66 ± 0.59 mg/dL), ALP (11.85 ± 0.99 U/L), GOT (3938.23 ± 251.41 U/L) and GPT (34.16 ± 4.93), decreased MDA levels in seminal tissue, and elevated the serum testosterone in the T2DM rats. Treatment with DSF ameliorated histological damage, significantly increased seminal 44 and 31 kDa TyrPho protein expression, and decreased that of caspase 3 and 9.
DSF extract was able to mitigate seminal dysfunction in T2DM rats via improvements of tyrosine phosphorylation, testosterone level and biochemical substances, as well as reductions of caspase proteins. DSF may be developed as an alternative medicine in treating of T2DM male subfertility and progressive complications.
尽管（Wall. ex DC.）西美烟（Bignoniaceae）花（DSF）可改善 2 型糖尿病动物的高血糖、睾丸损伤和精子质量，但它对产生精液的精囊的影响尚不清楚。
本研究报告了 DSF 对 2 型糖尿病大鼠精液功能障碍的保护作用。
雄性 Sprague-Dawley 大鼠分为四组（对照组、2 型糖尿病组、2 型糖尿病+DSF200 组和 2 型糖尿病+DSF600 组；每组 10 只）。对照组大鼠先给予低脂饮食 14 天，然后单次注射生理盐水；2 型糖尿病组大鼠给予高脂饮食和链脲佐菌素（50mg/kg 体重）注射。2 型糖尿病诱导大鼠连续 28 天经口给予 DSF（200 和 600mg/kg 体重）。实验结束时，评估血清生化成分、丙二醛（MDA）、组织学和精液裂解物中的蛋白质表达。
DSF 增加了血清磷（13.66±0.59mg/dL）、碱性磷酸酶（11.85±0.99U/L）、天门冬氨酸氨基转移酶（3938.23±251.41U/L）和丙氨酸氨基转移酶（34.16±4.93U/L）的水平，降低了精囊组织中的 MDA 水平，并提高了 2 型糖尿病大鼠的血清睾酮水平。DSF 治疗改善了组织学损伤，显著增加了 TyrPho 蛋白的 44 和 31kDa 表达，降低了 caspase3 和 9 的表达。
DSF 提取物通过改善酪氨酸磷酸化、睾酮水平和生化物质，以及降低半胱氨酸天冬氨酸蛋白酶蛋白，减轻 2 型糖尿病大鼠的精液功能障碍。DSF 可能被开发为治疗 2 型糖尿病男性不育和进行性并发症的替代药物。
