Bottomley S, Lovell-Smith C, Garcia-Webb P
Department of Clinical Biochemistry, Queen Elizabeth II Medical Centre, Nedlands, Perth, Western Australia.
J Steroid Biochem. 1988 Jan;29(1):145-8. doi: 10.1016/0022-4731(88)90388-3.
Incubating isolated rat hepatocytes with tritium labelled and unlabelled corticosterone at 37 degrees C resulted in the rapid appearance of at least nine corticosterone metabolites. A quick, relatively easy, and quantitative high performance liquid chromatographic (HPLC) method was used to separate these metabolites and follow their rate of appearance both intra- and extracellularly. We found different intra- and extracellular amounts of each metabolite at a particular time and this suggested that some metabolites were more available for transport than others.
将分离出的大鼠肝细胞与氚标记和未标记的皮质酮在37摄氏度下孵育，结果迅速出现了至少九种皮质酮代谢产物。采用一种快速、相对简便且定量的高效液相色谱（HPLC）方法来分离这些代谢产物，并追踪它们在细胞内和细胞外的出现速率。我们发现在特定时间每种代谢产物在细胞内和细胞外的量不同，这表明某些代谢产物比其他代谢产物更易于运输。
