Suprun Maria, Ellis Randall J, Sampson Hugh A, Suárez-Fariñas Mayte
Department of Pediatrics, Allergy and Immunology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Departments of Neuroscience and Psychiatry, Addiction Institute of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Bioinformatics. 2021 Apr 9;37(1):131-133. doi: 10.1093/bioinformatics/btaa1064.
Analysis of epitope-specific antibody repertoires has provided novel insights into the pathogenesis of inflammatory disorders, especially allergies. A novel multiplex immunoassay, termed Bead-Based Epitope Assay (BBEA), was developed to quantify levels of epitope-specific immunoglobulins, including IgE, IgG, IgA and IgD isotypes. bbeaR is an open-source R package, developed for the BBEA, provides a framework to import, process and normalize .csv data files exported from the Luminex reader, evaluate various quality control metrics, analyze differential epitope-binding antibodies with linear modeling, visualize results and map epitopes' amino acid sequences to their respective primary protein structures. bbeaR enables streamlined and reproducible analysis of epitope-specific antibody profiles.
bbeaR is open-source and freely available from GitHub as an R package: https://github.com/msuprun/bbeaR; vignettes included.
Supplementary data are available at Bioinformatics online.
表位特异性抗体库分析为炎症性疾病，尤其是过敏症的发病机制提供了新的见解。一种名为基于微珠的表位分析（BBEA）的新型多重免疫分析方法被开发出来，用于定量表位特异性免疫球蛋白的水平，包括IgE、IgG、IgA和IgD同种型。bbeaR是一个为BBEA开发的开源R包，提供了一个框架，用于导入、处理和标准化从Luminex读数仪导出的.csv数据文件，评估各种质量控制指标，用线性模型分析差异表位结合抗体，可视化结果，并将表位的氨基酸序列映射到它们各自的一级蛋白质结构。bbeaR能够对表位特异性抗体谱进行简化且可重复的分析。
bbeaR是开源的，可从GitHub上作为R包免费获取：https://github.com/msuprun/bbeaR；包含 vignettes。
补充数据可在《生物信息学》在线获取。
