利用 FLP 介导的定点基因整合技术在水稻中的应用。
FLP-Mediated Site-Specific Gene Integration in Rice.
机构信息
Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR, USA.
Department of Horticulture, University of Arkansas, Fayetteville, AR, USA.
出版信息
Methods Mol Biol. 2021;2238:231-240. doi: 10.1007/978-1-0716-1068-8_15.
Enabling precise gene integration is important for installing traits in the plants. One of the practical methods of achieving precise gene integration is by using the yeast FLP-FRT recombination system that is efficient in directing DNA integration into the "engineered" genomic sites. The critical parameters of this method include the use of the thermostable version of FLP protein and the promoter trap design to select site-specific integration clones. The resulting transgenic plants display stable expression that is transmitted to the progeny. Therefore, FLP-mediated site-specific integration method could be used for trait engineering in the crop plants or testing gene functions in the model plants.
实现精确的基因整合对于在植物中安装性状很重要。实现精确基因整合的一种实用方法是使用酵母 FLP-FRT 重组系统，该系统能够有效地将 DNA 整合到“工程化”的基因组位点中。该方法的关键参数包括使用热稳定的 FLP 蛋白和启动子陷阱设计来选择特异性整合克隆。产生的转基因植物表现出稳定的表达，并传递给后代。因此，FLP 介导的特异性整合方法可用于作物植物的性状工程或模型植物的基因功能测试。
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