Radhakrishnan Parthiban, Srivastava Vibha
Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR, 72701, USA.
Plant Cell Rep. 2005 Mar;23(10-11):721-6. doi: 10.1007/s00299-004-0876-x. Epub 2004 Oct 9.
To develop an FLP-FRT recombination system- (derived from 2 mu plasmid of Saccharomyces cerevisiae) based marker gene removal application for rice, we introduced the gene for FLP recombinase, under the control of the maize ubiquitin-1 promoter, into the rice genome. FLP activity was monitored in callus and regenerated plants by an assay based on the deletion of the FRT-flanked DNA fragment, leading to the activation of the beta-glucuronidase gene. FLP activity was detected both in the callus and leaves of some of the transgenic lines. Based on our comparison of the recombination efficiency of the FLP-FRT system expressed in the transgenic lines with that of the widely used Cre-lox system (derived from bacteriophage P1), we suggest that the FLP-FRT system is a useful tool for the genetic manipulation of rice.
为开发一种基于FLP - FRT重组系统（源自酿酒酵母的2μm质粒）的水稻标记基因去除应用，我们将受玉米泛素-1启动子控制的FLP重组酶基因导入水稻基因组。通过基于FRT侧翼DNA片段缺失的检测方法，在愈伤组织和再生植株中监测FLP活性，该缺失导致β-葡萄糖醛酸酶基因的激活。在一些转基因株系的愈伤组织和叶片中均检测到了FLP活性。基于我们对转基因株系中表达的FLP - FRT系统与广泛使用的Cre - lox系统（源自噬菌体P1）重组效率的比较，我们认为FLP - FRT系统是水稻遗传操作的一种有用工具。
