School of Food Science and Biotechnology, Zhejiang Gongshang University, Xiasha, Hangzhou, 310018, P. R. China.
Department of Food Science, Faculty of Hospitality Management, Shanghai Business School, Shanghai, 200235, P. R. China.
Chem Biodivers. 2021 Mar;18(3):e2000930. doi: 10.1002/cbdv.202000930. Epub 2021 Feb 10.
Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4. These components were characterized by chemical composition assay, GC/MS, HPGPC, UV and FT-IR techniques. The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power. The results suggested that all the four components possess good antioxidant activities. Among them, ESFP1 was found to possess the strongest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical-scavenging activity, and the greatest ferric reducing power. The immunomodulatory effect of these four polysaccharides was demonstrated by their ability to promote proliferation, and to enhance both phagocytic activity and NO release in a macrophage RAW264.7 model. The results revealed that the bioactivities of the polysaccharides are related to their molecular weight, and the uronic acid and sulfate contents.
从马尾藻中提取的粗多糖（SFP）的酶解产物经柱 DEAE-52 和 Sephadex G-100 纯化，得到四个组分，分别为 ESFP1、ESFP2、ESFP3 和 ESFP4。这些组分通过化学组成分析、GC/MS、HPGPC、UV 和 FT-IR 技术进行了表征。通过测量自由基清除活性和还原能力，研究了这四个纯化部分的体外抗氧化活性。结果表明，所有四个组分均具有良好的抗氧化活性。其中，ESFP1 被发现具有最强的 1,1-二苯基-2-苦基肼基（DPPH）自由基和羟基自由基清除活性，以及最大的铁还原能力。通过促进增殖以及增强巨噬细胞 RAW264.7 模型中的吞噬活性和 NO 释放来证明这四种多糖的免疫调节作用。结果表明，多糖的生物活性与其分子量、糖醛酸和硫酸基含量有关。
