Marudhupandi T, Kumar T T Ajith, Senthil S Lakshmana, Devi K Nanthini
Pak J Biol Sci. 2014 Feb 1;17(3):402-7. doi: 10.3923/pjbs.2014.402.407.
The aim of the present study is conducted to evaluate the antioxidant potential and toxicity effect of polysaccharide-fucoidan from the brown seaweed Sargassum tenerrimum. Fucoidan-a sulphated polysaccharide contains fucose and sulphate as major compounds. Interestingly, various studies reported that the presence of sulphate content of the sample plays a significant role in pharmacological activities. In this study, fucoidan was fractionated by Ion exchange chromatography method and it major chemical constituent sulphate and fucose was determined by the biochemical methods. The toxicity effect of fucoidan was analyzed by the brine shrimp toxicity assay. Three fucoidan fractions (F1, F2 and F3) were obtained from Intact Fucoidan (IF) through anion-exchange column chromatography. In vitro antioxidant capability was analyzed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radical scavenging and total antioxidant assays and intact fucoidan showed the maximum activity 83.66 +/- 0.35, 81.73 +/- 0.35% and 41.6 +/- 0.43 mg g(-1), respectively. The finding of the present study was confirmed that the antioxidant property of fucoidan was depending upon the sulphate content of the fraction and these studies proved that fucoidan have non toxicity effect. Hence, fucoidan have the scope of being used as natural antioxidants in treating many human diseases.
本研究旨在评估来自棕色海藻柔弱马尾藻的多糖岩藻聚糖的抗氧化潜力和毒性作用。岩藻聚糖是一种硫酸化多糖，主要成分是岩藻糖和硫酸盐。有趣的是，各种研究报告称，样品中硫酸盐含量的存在在药理活性中起着重要作用。在本研究中，岩藻聚糖通过离子交换色谱法进行分离，其主要化学成分硫酸盐和岩藻糖通过生化方法测定。岩藻聚糖的毒性作用通过卤虫毒性试验进行分析。通过阴离子交换柱色谱从完整岩藻聚糖（IF）中获得了三个岩藻聚糖级分（F1、F2和F3）。通过1,1-二苯基-2-苦基肼（DPPH）、超氧阴离子自由基清除和总抗氧化剂测定分析体外抗氧化能力，完整岩藻聚糖分别显示出最大活性83.66±0.35、81.73±0.35%和41.6±0.43 mg g(-1)。本研究结果证实，岩藻聚糖的抗氧化特性取决于级分中的硫酸盐含量，这些研究证明岩藻聚糖无毒性作用。因此，岩藻聚糖有作为天然抗氧化剂用于治疗多种人类疾病的应用前景。
