角蛋白-8 和角蛋白-18 多态性及其蛋白水平在声带白斑发生和进展中的作用。

The Role of Keratin-8 and Keratin-18 Polymorphisms and Protein Levels in the Occurrence and Progression of Vocal Leukoplakia.

机构信息

Department of Otolaryngology-Head and Neck Surgery, Eye and ENT Hospital, Fudan University, Shanghai, China.

Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye and ENT Hospital, Fudan University, Shanghai, China.

出版信息

ORL J Otorhinolaryngol Relat Spec. 2021;83(2):65-74. doi: 10.1159/000511447. Epub 2021 Jan 20.

DOI:10.1159/000511447

PMID:33472210

Abstract

OBJECTIVE

This study aimed to evaluate the association between the single-nucleotide polymorphism (SNP) and tissue protein level of keratin-8/18 and the occurrence and progression of vocal leukoplakia.

METHODS

The case-control study enrolled 158 patients with vocal leukoplakia, 326 patients with laryngeal squamous cell carcinoma (LSCC), and 268 healthy controls, which were tested for genotype analysis with keratin-8 and keratin-18 gene polymorphisms using pyrosequencing. The tissue protein expression levels of keratin-8 and keratin-18 were evaluated using immunohistochemistry.

RESULTS

The keratin-8 SNP RS1907671 showed an obvious increased risk for vocal leukoplakia (OR 1.56, p = 0.002), while the other SNPs (RS2035875, RS2035878, RS4300473) were tested as protective factors for vocal leukoplakia and LSCC (OR <1, p < 0.05). In keratin-18 SNP test, both RS2070876 and RS2638526 polymorphisms demonstrated decreased risks for vocal leukoplakia and LSCC (OR <1, p < 0.05). The protein levels of keratin-8 and keratin-18 in vocal leukoplakia group were significantly higher than those of the LSCC group (p < 0.05).

CONCLUSIONS

Keratin-8 and keratin-18 polymorphisms and protein levels are associated with the occurrence and progression of vocal leukoplakia.

摘要

目的

本研究旨在评估角蛋白-8/18 单核苷酸多态性（SNP）与组织蛋白水平与声带白斑发生和进展的关系。

方法

采用病例对照研究，纳入 158 例声带白斑患者、326 例喉鳞状细胞癌（LSCC）患者和 268 例健康对照者，采用焦磷酸测序法检测角蛋白-8 和角蛋白-18 基因多态性，进行基因型分析。采用免疫组织化学法检测角蛋白-8 和角蛋白-18 的组织蛋白表达水平。

结果

角蛋白-8 SNP RS1907671 对声带白斑的发生有明显的增高风险（OR 1.56，p = 0.002），而其他 SNP（RS2035875、RS2035878、RS4300473）则被测试为声带白斑和 LSCC 的保护因素（OR<1，p<0.05）。在角蛋白-18 SNP 检测中，RS2070876 和 RS2638526 多态性均显示对声带白斑和 LSCC 的风险降低（OR<1，p<0.05）。声带白斑组的角蛋白-8 和角蛋白-18 蛋白水平明显高于 LSCC 组（p<0.05）。