Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Road, Tai'an, 271018, Shandong Province, China.
Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, College of Basic Medical Sciences, Shandong First Medical University, Tai'an, 271000, Shandong Province, China.
Vet Res. 2021 Jan 20;52(1):9. doi: 10.1186/s13567-020-00889-4.
Novel duck reovirus (NDRV) causes severe economic losses to the duck industry, which is characterized by hemorrhagic spots and necrotic foci of the livers and spleens. DEAD-box helicase 1 (DDX1) plays a critical role in the innate immune system against viral infection. However, the role of duck DDX1 (duDDX1) in anti-RNA virus infection, especially in the anti-NDRV infection, has yet to be elucidated. In the present study, the full-length cDNA of duDDX1 (2223 bp encode 740 amino acids) was firstly cloned from the spleen of healthy Cherry valley ducks, and the phylogenetic tree indicated that the duDDX1 has the closest relationship with Anas platyrhynchos in the bird branch. The duDDX1 mRNA was widely distributed in all tested tissues, especially in the duodenum, liver, and spleen. Overexpression of duDDX1 in primary duck embryo fibroblast (DEF) cells triggered the activation of transcription factors IRF-7 and NF-κB, as well as IFN-β expression, and the expression of the Toll-like receptors (TLR2, TLR3, and TLR4) was significantly increased. Importantly, after overexpressing or knocking down duDDX1 and infecting NDRV in DEF cells, duDDX1 inhibits the replication of NDRV virus and also regulates the expression of pattern recognition receptors and cytokines. This indicates that duDDX1 may play an important role in the innate immune response of ducks to NDRV. Collectively, we first cloned DDX1 from ducks and analyzed its biological functions. Secondly, we proved that duck DDX1 participates in anti-NDRV infection, and innovated new ideas for the prevention and control of duck virus infection.
新型鸭呼肠孤病毒（NDRV）引起鸭业严重经济损失，其特征为肝脏和脾脏出现出血斑点和坏死灶。DEAD 框解旋酶 1（DDX1）在抗病毒感染的固有免疫系统中发挥关键作用。然而，鸭 DDX1（duDDX1）在抗 RNA 病毒感染中的作用，特别是在抗 NDRV 感染中的作用尚未阐明。本研究首次从健康樱桃谷鸭脾脏中克隆得到全长 duDDX1 cDNA（2223bp 编码 740 个氨基酸），系统进化树分析表明 duDDX1 在鸟类分支中与 Anas platyrhynchos 的亲缘关系最近。duDDX1mRNA 在所有检测的组织中广泛表达，特别是在十二指肠、肝脏和脾脏中。在原代鸭胚成纤维细胞（DEF）中过表达 duDDX1 可触发转录因子 IRF-7 和 NF-κB 的激活以及 IFN-β 的表达，并显著增加 Toll 样受体（TLR2、TLR3 和 TLR4）的表达。重要的是，在 DEF 细胞中转染过表达或敲低 duDDX1 并感染 NDRV 后，duDDX1 抑制 NDRV 病毒的复制，同时调节模式识别受体和细胞因子的表达。这表明 duDDX1 可能在鸭对 NDRV 的固有免疫反应中发挥重要作用。总之，我们首次从鸭中克隆了 DDX1 并分析了其生物学功能。其次，我们证明了鸭 DDX1 参与抗 NDRV 感染，为鸭病毒感染的防控提供了新的思路。
