Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
J Proteomics. 2019 Feb 20;193:162-172. doi: 10.1016/j.jprot.2018.10.006. Epub 2018 Oct 16.
Duck reovirus (DRV), a member of the genus Orthoreovirus in the family Reoviridae, was first isolated from Muscovy ducks. The disease associated with DRV causes great economic losses to the duck industry. However, the responses of duck (Cairna moschata) to the classical/novel DRV (C/NDRV) infections are largely unknown. To reveal the relationship of pathogenesis and immune response, the proteomes of duck spleen cells under the control and C/NDRV infections were compared. In total, 5986 proteins were identified, of which 5389 proteins were quantified. The different accumulated proteins (DAPs) under the C/NDRV infections showed displayed various biological functions and diverse subcellular localizations. The proteins related to the serine protease system were siginificantly changed, suggesting that the activated serine protease system may play an important role under the C/NDRV infections. Furthermore, the differences in the responses to the C/NRDV infections between the duck liver and spleen tissues were compared. Only a small number of common DAPs were identified in both liver and spleen tissues, suggesting diversified pattern involved in the responses to the C/NRDV infections. However, the changes in the proteins involved in the serine protease systems were similar in both liver and spleen cells. Our data may give a comprehensive resource for investigating the responses to C/NDRV infections in ducks. SIGNIFICANCE: A newly developed MS/MS-based method involving isotopomer labels and 'tandem mass' has been applied to protein accurate quantification in current years. However, no studies on the responses of duck (Cairna moschata) spleen tissue to the classical/novel DRV (C/NDRV) infections have been performed. As a continued study of our previous report on the responses of duck liver tissue to the C/NDRV infections, the current study further compared the differences in the responses to the C/NRDV infections between the duck liver and spleen tissues. Our results will provide an opportunity to reveal the relationship of pathogenesis and immune response and basic information on the pathogenicity of C/NDRV in ducks.
鸭呼肠孤病毒（DRV），呼肠孤病毒科呼肠孤病毒属的成员，最初从麝香鸭中分离出来。与 DRV 相关的疾病给养鸭业造成了巨大的经济损失。然而，鸭（Cairna moschata）对经典/新型 DRV（C/NDRV）感染的反应在很大程度上尚不清楚。为了揭示发病机制和免疫反应的关系，比较了对照和 C/NDRV 感染下鸭脾细胞的蛋白质组。共鉴定出 5986 种蛋白质，其中 5389 种蛋白质被定量。C/NDRV 感染下不同积累的蛋白质（DAP）显示出各种生物学功能和不同的亚细胞定位。与丝氨酸蛋白酶系统相关的蛋白质发生了显著变化，表明激活的丝氨酸蛋白酶系统在 C/NDRV 感染下可能发挥重要作用。此外，还比较了鸭肝和脾组织对 C/NRDV 感染的反应差异。在肝和脾组织中仅鉴定出少数共同的 DAP，表明在对 C/NRDV 感染的反应中存在多样化的模式。然而，参与丝氨酸蛋白酶系统的蛋白质变化在肝和脾细胞中相似。我们的数据可能为研究鸭对 C/NDRV 感染的反应提供了全面的资源。意义：近年来，一种新开发的基于 MS/MS 的方法，涉及同位素标记和“串联质谱”，已被应用于蛋白质的精确定量。然而，目前还没有研究鸭（Cairna moschata）脾脏组织对经典/新型 DRV（C/NDRV）感染的反应。作为我们之前关于鸭肝组织对 C/NDRV 感染反应的报告的延续性研究，本研究进一步比较了鸭肝和脾组织对 C/NRDV 感染反应的差异。我们的研究结果将提供一个机会，以揭示发病机制和免疫反应之间的关系，以及 C/NDRV 在鸭中的致病性的基础信息。
