College of Veterinary Medicine, Northwest A&F University, Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling 712100, Shaanxi Province, China.
School of Life Science and Technology, Harbin Institute of Technology, Science Park of Harbin Institute of Technology, Harbin 150000, China.
Development. 2021 Mar 5;148(5):dev189670. doi: 10.1242/dev.189670.
In mammals, sperm-borne regulators can be transferred to oocytes during fertilization and have different effects on the formation of pronuclei, the first cleavage of zygotes, the development of preimplantation embryos and even the metabolism of individuals after birth. The regulatory role of sperm microRNAs (miRNAs) in the development of bovine preimplantation embryos has not been reported in detail. By constructing and screening miRNA expression libraries, we found that miR-202 was highly enriched in bovine sperm. As a target gene of miR-202, co-injection of siRNA can partially reverse the accelerated first cleavage of bovine embryos caused by miR-202 inhibitor. In addition, both a miR-202 mimic and siRNA delayed the first cleavage of somatic cell nuclear transfer (SCNT) embryos, suggesting that miR-202- mediates the delay of first cleavage of bovine embryos. By further exploring the relationship between miR-202/SEPT7, HDAC6 and acetylated α-tubulin during embryonic development, we investigated how sperm-borne miR-202 regulates the first cleavage process of bovine embryos by and demonstrate the potential of sperm-borne miRNAs to improve the efficiency of SCNT.
在哺乳动物中，精子携带的调控因子可在受精过程中传递到卵母细胞，并对原核形成、受精卵第一次分裂、胚胎着床前发育甚至个体出生后的代谢产生不同的影响。精子 microRNAs (miRNAs) 在牛胚胎着床前发育中的调控作用尚未有详细报道。通过构建和筛选 miRNA 表达文库，我们发现 miR-202 在牛精子中高度富集。作为 miR-202 的靶基因，siRNA 的共注射可以部分逆转 miR-202 抑制剂引起的牛胚胎第一次分裂加速。此外，miR-202 模拟物和 siRNA 均延迟了体细胞核移植 (SCNT) 胚胎的第一次分裂，表明 miR-202 介导了牛胚胎第一次分裂的延迟。通过进一步探讨 miR-202/SEPT7、HDAC6 和乙酰化α-微管蛋白在胚胎发育过程中的关系，我们研究了精子携带的 miR-202 如何通过调控 SEPT7 和 HDAC6 来调节牛胚胎的第一次分裂过程，并证明了精子携带的 miRNAs 提高 SCNT 效率的潜力。
