Li Sha, Liu Xueqing, Bai Yunqin, Huang Tao, Wang Junhong, Qu Huantao, Chen Lei, Jiang Wei
Institute of Chinese Sturgeon, China Three Gorges Corporation, Yichang, China.
Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Institute of Chinese Sturgeon, China Three Gorges Corporation, Yichang, China.
Mitochondrial DNA B Resour. 2016 Feb 1;1(1):39-40. doi: 10.1080/23802359.2015.1137812.
The complete mitochondrial genome sequence of the hybrid of was first determined by a PCR-based sequencing method in this study. The mitochondrial was 16 624 bp in length, including 13 protein genes, two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and one control region. All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. Base composition of the heavy strain was A (30.09%), T (24.96%), C (27.67%), G (16.41%) and with A + T bias of 55.05%. All the genes in were distributed on the H-strand, except for the subunit gene and nine tRNA genes which were encoded on the L-strand. The phylogenetic relationships of 14 Cyprinidae species were reconstructed based on the complete mtDNA sequences using the MEGA inference method.
本研究首次采用基于聚合酶链反应（PCR）的测序方法测定了该杂交种的完整线粒体基因组序列。线粒体长度为16624碱基对（bp），包括13个蛋白质基因、两个核糖体RNA（rRNA）基因、22个转运RNA（tRNA）基因和一个控制区。除ND6和8个tRNA基因外，所有基因均在重链上编码。重链的碱基组成为：腺嘌呤（A，30.09%）、胸腺嘧啶（T，24.96%）、胞嘧啶（C，27.67%）、鸟嘌呤（G，16.41%），A+T偏差为55.05%。除了亚基基因和9个在轻链上编码的tRNA基因外，所有基因均分布在重链上。基于完整的线粒体DNA序列，采用MEGA推断方法重建了14种鲤科鱼类的系统发育关系。
