Zhang Jing, Liu Tao, Zhang Lei, Liang Jing
Qilu University of Technology, Jinan, People's Republic of China.
College of Marine Life Sciences, Ocean University of China, Qingdao, People's Republic of China.
Mitochondrial DNA B Resour. 2016 Jun 20;1(1):406-407. doi: 10.1080/23802359.2016.1176878.
Complete mitogenome of variety 'Ailunwan' ( ×) was obtained in this work. Circular-mapping mitogenome was 37,657 bp with overall A + T content of 64.71%, encoding 3 rRNAs (23S, 16S and 5S), 25 tRNAs, 35 protein-coding genes and 3 open reading frames (ORFs). Gene content and genome organization of 'Ailunwan' mitogenome were identical to those species. From the total alignment of 'Ailunwan' and another reported variety 'Rongfu' ( ×), 33 nucleotide substitutions were detected, and one intergenic region of 19 nucleotides insertion/deletion was found which located between 3 and 9. The phylogenetic analysis based on mitogenomes showed that 'Ailunwan' had a closer evolutionary relationship with than and validated the genus and were polyphyletic.
本研究获得了‘爱伦湾’(×)品种的完整线粒体基因组。环状线粒体基因组为37,657 bp,A+T总含量为64.71%,编码3个rRNA(23S、16S和5S)、25个tRNA、35个蛋白质编码基因和3个开放阅读框(ORF)。‘爱伦湾’线粒体基因组的基因含量和基因组组织与那些物种相同。通过‘爱伦湾’与另一个已报道品种‘荣福’(×)的全序列比对,检测到33个核苷酸替换,并且发现一个19个核苷酸的插入/缺失的基因间区域,其位于3和9之间。基于线粒体基因组的系统发育分析表明,‘爱伦湾’与[具体品种1]的进化关系比与[具体品种2]更近,并验证了[属名1]属和[属名2]属是多系的。 (注:原文中部分品种名、属名等未明确给出具体内容,翻译时用[具体品种名]、[具体属名]等表示)