双壳底栖硅藻（舟形藻目，硅藻纲）的完整线粒体基因组
Complete mitochondrial genome of biraphid benthic diatom, (Naviculales, Bacillariophyceae).
作者信息
An Sung Min, Noh Jae Hoon, Lee Hyee Ryun, Choi Dong Han, Lee Jung Ho, Yang Eun Chan
机构信息
Marine Ecosystem and Biological Research Center, Korea Institute of Ocean Science & Technology, Ansan, Korea.
Department of Biology Education, Daegu University, Gyeongsan, Korea.
出版信息
Mitochondrial DNA B Resour. 2016 Jul 23;1(1):549-550. doi: 10.1080/23802359.2016.1198997.
The complete mitochondrial DNA of biraphid benthic diatom, TA439 was sequenced and characterized. The circular mitogenome contains 67 genes in 48,652 bp (31.1% GC), including 41 protein-coding, 24 transfer RNA (tRNA) and 2 rRNA genes. Twenty-four protein-coding sequences (CDS, 59%) have start with ATG codon and 17 CDS start with alternatives such as ATA (5), ATT (6), TTA (5) and TTG (1). The GC content of tRNA genes (42.1%) is relatively higher than those of the rRNA (35.2%) and CDS (30.5%). Three genes are consisted of multiple exons and introns, i.e. (three exons, two introns), (two exons, one intron), (four exons, three introns). Phylogeny of diatoms based on mitogenome data (34 CDS, 8530 amino acids combined) supports the monophyly of Naviculales, including (Naviculaceae), (Berkeleyaceae), (Stauroneidaceae) and (Phaeodactylaceae). Mitogenome data may be useful for phylogenetic study of the diatoms and stramenopiles.
对双壳底栖硅藻TA439的完整线粒体DNA进行了测序和特征分析。环状线粒体基因组包含67个基因，长度为48,652 bp（GC含量为31.1%），包括41个蛋白质编码基因、24个转运RNA（tRNA）基因和2个核糖体RNA（rRNA）基因。24个蛋白质编码序列（CDS，占59%）以ATG密码子起始，17个CDS以ATA（5个）、ATT（6个）、TTA（5个）和TTG（1个）等替代密码子起始。tRNA基因的GC含量（42.1%）相对高于rRNA（35.2%）和CDS（30.5%）。三个基因由多个外显子和内含子组成，即（三个外显子，两个内含子）、（两个外显子，一个内含子）、（四个外显子，三个内含子）。基于线粒体基因组数据（共34个CDS，8530个氨基酸）构建的硅藻系统发育树支持舟形藻目为单系类群，包括（舟形藻科）、（伯克利藻科）、（星杆藻科）和（褐指藻科）。线粒体基因组数据可能有助于硅藻和不等鞭毛类的系统发育研究。
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