Resende Leonardo Cardoso, do Carmo Anderson Oliveira, Núñez-Rodriguez Daniela, Pimentel Juliana da Silva Martins, Bedore Alessandra Gomes, Leal Hortênsia Gomes, Kalapothakis Evanguedes
Biologia Geral, Universidade Federal de Minas Gerais Instituto de Ciencias Biologicas, Belo Horizonte, Brazil.
Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Mitochondrial DNA B Resour. 2016 Nov 22;1(1):806-808. doi: 10.1080/23802359.2016.1219646.
Pimelodus is an important commercial fish found in the São Francisco and Paraná river basins. NGS was used to sequence the mtDNA of . The mtDNA was annotated and aligned with that of 25 other fish species to enable phylogenetic analysis. The complete mtDNA molecule had 16,561 bp and its GC content was 43.7%; the structure was similar to that of other vertebrates: 2 rRNA, 22 tRNA, 13 protein-coding genes, and a D-loop region containing 914 bp. Phylogenetic analysis yielded a tree with a high bootstrap coefficient that was coherent with the actual phylogeny of the species.
长须鲶是一种在圣弗朗西斯科河和巴拉那河流域发现的重要商业鱼类。利用二代测序技术（NGS）对其线粒体DNA（mtDNA）进行测序。对该mtDNA进行注释，并与其他25种鱼类的mtDNA进行比对，以进行系统发育分析。完整的mtDNA分子有16,561个碱基对，其GC含量为43.7%；其结构与其他脊椎动物相似：2个核糖体RNA、22个转运RNA、13个蛋白质编码基因，以及一个包含914个碱基对的控制区（D-loop区）。系统发育分析得出了一棵具有高自展系数的树，该树与该物种的实际系统发育情况一致。
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