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斑点叉尾鮰微卫星位点的分离与特性分析(鲇形目:鮰科)。

Isolation and characterization of microsatellite loci in Pimelodus maculatus (Siluriformes: Pimelodidae).

机构信息

Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

出版信息

Mol Ecol Resour. 2008 Sep;8(5):1078-80. doi: 10.1111/j.1755-0998.2008.02160.x. Epub 2008 Jun 28.

Abstract

To evaluate genetic variability in Pimelodus maculatus, a small migratory catfish common in South America, we isolated and characterized several microsatellite markers from individuals collected from a population in the River Grande (Brazil). A total of 11 loci were obtained and analyzed. All the loci were polymorphic, with the number of alleles ranging from three to 27 per locus. Observed and expected heterozygosities ranged from 0.5 to 0.89 and from 0.53 to 0.95, respectively. These polymorphic markers can be used as effective tools to study the genetic structure in populations of this species.

摘要

为了评估南美白对虾(Pimelodus maculatus)的遗传变异性,我们从格兰德河(巴西)的一个种群中分离并鉴定了来自个体的几个微卫星标记。共获得并分析了 11 个位点。所有的位点都是多态的,每个位点的等位基因数从三个到 27 个不等。观察到的和预期的杂合度分别在 0.5 到 0.89 和 0.53 到 0.95 之间。这些多态性标记可作为研究该物种种群遗传结构的有效工具。

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