Eimanifar Amin, T Kimball Rebecca, L Braun Edward, Ellis James D
Honey Bee Research and Extension Laboratory, Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA.
Department of Biology, University of Florida, Gainesville, FL, USA.
Mitochondrial DNA B Resour. 2016 Nov 11;1(1):856-857. doi: 10.1080/23802359.2016.1250132.
We characterized the complete mitogenome sequence of the South African hybrid honey bee × using genome skimming. The mitochondrial genome was a circular molecule 16,340 bp in length with a gene organization identical to that of the other mitogenomes. The base composition is 43.2% A, 9.7% C, 5.6% G, and 41.5% T, with an A + T content of 84.7%. The mitogenome had 13 protein-coding genes (PCGs), 22 transfer RNAs, two ribosomal RNAs genes, and one control region. All PCGs were initiated by ATT, ATG, ATA, and ATC codons and were terminated by a TAA stop codon. The heavy strand encodes four PCGs, eight tRNAs, and two rRNAs. The light strand encodes nine PCGS and 14 tRNAs. A phylogenetic analysis of the PCGs reveals a close relationship between this hybrid honey bee and other spp.
我们通过基因组鸟枪法对南非杂交蜜蜂×的完整线粒体基因组序列进行了表征。线粒体基因组是一个长度为16340 bp的环状分子,其基因组织与其他线粒体基因组相同。碱基组成为43.2%A、9.7%C、5.6%G和41.5%T,A+T含量为84.7%。线粒体基因组有13个蛋白质编码基因(PCG)、22个转运RNA、2个核糖体RNA基因和1个控制区。所有PCG均由ATT、ATG、ATA和ATC密码子起始,并由TAA终止密码子终止。重链编码4个PCG、8个tRNA和2个rRNA。轻链编码9个PCG和14个tRNA。对PCG的系统发育分析表明,这种杂交蜜蜂与其他 spp. 之间关系密切。