Komissarov Aleksey, Korchagin Vitaly, Kliver Sergei, Dobrynin Pavel, Semyenova Serafima, Vergun Andrey, O'Brien Stephen, Ryskov Alexey
Theodosius Dobzhansky Center for Genome Bioinformatics Saint Petersburg State University, St. Petersburg, Russia.
Laboratory of Genome Organization, Institute of Gene Biology of the Russian Academy of Sciences, Moscow, Russia.
Mitochondrial DNA B Resour. 2016 Dec 9;1(1):875-877. doi: 10.1080/23802359.2016.1253040.
The first complete mitochondrial genome sequence of parthenogenetic Caucasian rock lizard s ( family) is determined by hybrid assembly with Illumina HiSeq and PacBio RS II platforms. The circular 21.4 kbp mitogenome contains 13 protein-coding genes, 12S and 16S rRNA genes, 20 tRNAs, two pseudogenized tRNAs, and one long tandem repeats with 4.1 kbp length formed by 59 bp monomer repeated x70.6 times located before control region. This finding represents a new example of mitogenome variation in lizards of hybrid origin, providing fundamental data for following study of a unique hybridization system formed by parthenogenetic and bisexual species in the mountain steppe of central Armenia.
通过Illumina HiSeq和PacBio RS II平台的混合组装,确定了孤雌生殖的高加索岩蜥(家族)的首个完整线粒体基因组序列。这个21.4 kbp的环状线粒体基因组包含13个蛋白质编码基因、12S和16S rRNA基因、20个tRNA、两个假基因化的tRNA,以及一个位于控制区之前的长度为4.1 kbp的长串联重复序列,该序列由59 bp的单体重复70.6次形成。这一发现代表了杂交起源蜥蜴线粒体基因组变异的一个新例子,为后续研究亚美尼亚中部山地草原孤雌生殖和两性物种形成的独特杂交系统提供了基础数据。