Fangliang Huang, Yongquan Li, Xinai Chen
College of Life Sciences, Zhejiang University, Hangzhou, China.
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
Mitochondrial DNA B Resour. 2017 Feb 16;2(1):95-96. doi: 10.1080/23802359.2017.1285208.
As part of a genome sequencing project for (), a complete mitochondrial (mt) genome was assembled as a single circular dsDNA of 35,159 bp. Conserved genes including the large and small rRNA subunits, 25 tRNA and 15 protein-coding genes, were identified. In addition, 4 non-conserved open reading frames (ncORFs) in the intergenic and intronic regions were also identified. Transcription analyses using RNA-seq validated the expression of most conserved genes and ncORFs. Seven introns (groups I and II) were found within conserved genes, accounting for 21% of the mitogenome. All structural genes are located on one strand and are apparently transcribed in one direction. The complete mt genomes of would be useful for future investigation of genetics, evolution and clinical identification of species.
作为（）基因组测序项目的一部分，一个完整的线粒体（mt）基因组被组装成一个35,159 bp的单环双链DNA。鉴定出了包括大、小rRNA亚基、25个tRNA和15个蛋白质编码基因在内的保守基因。此外，还在基因间区和内含子区鉴定出4个非保守开放阅读框（ncORFs）。使用RNA-seq进行的转录分析验证了大多数保守基因和ncORFs的表达。在保守基因中发现了7个内含子（I组和II组），占线粒体基因组的21%。所有结构基因都位于一条链上，显然是按一个方向转录的。（）的完整线粒体基因组将有助于未来对（）物种的遗传学、进化和临床鉴定进行研究。
