Mandon Perrine, Aznar-Cormano Laetitia, Hourdez Stéphane, Samadi Sarah
Muséum National d'Histoire Naturelle, Sorbonne Universités, Institut de Systématique, Evolution, Biodiversité, (ISYEB - UMR 7205 - CNRS, MNHN, UPMC, EPHE), Paris, France.
Sorbonne Universités, UPMC Univ Paris 6, Station Biologique de Roscoff, Roscoff, France.
Mitochondrial DNA B Resour. 2017 May 19;2(1):291-293. doi: 10.1080/23802359.2017.1318674.
We assembled the mitogenome of the Bythograeid crab , using long-range amplification of the mitochondrial genome. The mitogenome is 15,521 base pair long (33.8% A, 21.7% C, 10.5% G, 34% T) with 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs, and a 624 bp AT-rich region. The gene arrangement is similar to other Brachyuran species. A whole genome shotgun sequencing approach revealed the presence of mitochondrial pseudogenes in the nuclear genome. This fifth mitogenome for a species of Bythograeidae should help resolve the puzzling question of the evolutionary origin of a family limited to deep-sea hydrothermal vents.
我们利用线粒体基因组的长距离扩增技术，组装了深海蟹类的线粒体基因组。该线粒体基因组长度为15,521个碱基对（A占33.8%，C占21.7%，G占10.5%，T占34%），包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA以及一个624 bp的富含AT区域。基因排列与其他短尾类物种相似。全基因组鸟枪法测序方法揭示了核基因组中存在线粒体假基因。这一深海蟹科物种的第五个线粒体基因组应有助于解决一个仅限于深海热液喷口的科的进化起源这一令人困惑的问题。
