Kim Se-Joo, Moon Jai-Woon, Ju Se-Jong
a Korean Bioinformation Center, Korea Research Institute Bioscience and Biotechnology, Daejeon , Republic of Korea .
b Deep-sea and Seabed Mineral Resources Research Center, Korea Institute of Ocean Science & Technology , Gyeonggi-do , Republic of Korea , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2719-20. doi: 10.3109/19401736.2015.1046162. Epub 2015 Jun 9.
The brachyuran crab Gandalfus puia is a species endemic to the hydrothermal vent fields in the Tonga-Kermadec Arc. In order to understand G. puia at the genomic level, we sequenced its mitochondrial genome (mitogenome) and then compared to other bythograeids. The mitogenome is 15,548 bp in length and exhibits brachyuran-typical gene arrangement. Its protein-coding genes were very similar to other bythograeid species with respect to length, AT content and start and stop codons. Additionally, we compared the mitogenomes of Gandalfus and the closely related Austinograea. The inter-specific nucleotide divergence was 13.4% in Gandalfus and 13.7-14.0% in Austinograea. The inter-generic nucleotide divergence between Gandalfus and Austinograea was 16.3-19.7%. Based on the phylogenetic tree constructed with maximum likelihood and Bayesian inference, bythograeid crabs were recognized as the monophyletic taxon with the high supporting values (100% bootstrap proportions and 1.00 posterior probabilities). These results are useful for understanding the phylogenetic relationships and evolution of bythograeid crabs.
短尾蟹Gandalfus puia是汤加-克马德克海沟热液喷口区特有的物种。为了在基因组水平上了解G. puia，我们对其线粒体基因组（有丝分裂基因组）进行了测序，然后与其他深海蟹科物种进行比较。该有丝分裂基因组长度为15548 bp，呈现出短尾蟹典型的基因排列。其蛋白质编码基因在长度、AT含量以及起始和终止密码子方面与其他深海蟹科物种非常相似。此外，我们比较了Gandalfus和近缘的奥氏深海蟹（Austinograea）的有丝分裂基因组。Gandalfus种间核苷酸差异为13.4%，奥氏深海蟹为13.7%-14.0%。Gandalfus和奥氏深海蟹之间的属间核苷酸差异为16.3%-19.7%。基于最大似然法和贝叶斯推断构建的系统发育树，深海蟹科蟹类被认为是具有高支持值（100%自展比例和1.00后验概率）的单系类群。这些结果有助于了解深海蟹科蟹类的系统发育关系和进化。
