Valero M Carmen, Ojo James Adebayo, Sun Weilin, Tamò Manuele, Coates Brad S, Pittendrigh Barry R
Carl R. Woese Institute of Genomic Biology, University of Illinois at Urbana-Champaign, IL, USA.
Department of Crop Production, Kwara State University, Ilorin, Nigeria.
Mitochondrial DNA B Resour. 2017 Jul 18;2(2):421-423. doi: 10.1080/23802359.2017.1347829.
The complete 16,345-bp mitochondrial genome of the agriculturally destructive pod sucking pest, the giant coreid bug, (Hemiptera: Coreidae), was assembled from paired-end Illumina HiSeq 2500 reads. The mitochondrial genome consists of 13 protein coding genes (PCGs), 22 tRNAs, 2 rRNAs and a control region in the order and orientation typical among insects. PCG initiation codons (ATG, ATC, ATT and ATA) with termination codon (TAA) are used with the exception of TAG stop codons by Cytb and ND3. All tRNA genes fold into predicted cloverleaf secondary structures having requisite triplets on the anticodon loop, apart from tRNA-Ser1 (AGN) whose dihydrouridine (DHU) arm forms a simple loop. The phylogenetic analysis of hemipteran mitogenomes clusters to the family level and supports the monophyly of the five superfamilies in Pentatomomorpha of Hemiptera. The Coreoidea and Pyrrhocoroidea are sister groups, while Coreidae and Alydidae are sister groups to Rhopalidae. These analyses provide insight to mitogenomics and evolutionary relationships among pentatomoid insects.
利用成对末端Illumina HiSeq 2500测序数据，组装得到了对农业具有破坏性的吸食豆荚害虫——大缘蝽（半翅目：缘蝽科）完整的16345碱基对线粒体基因组。该线粒体基因组由13个蛋白质编码基因（PCG）、22个tRNA、2个rRNA和一个控制区组成，其排列顺序和方向在昆虫中具有典型性。除细胞色素b（Cytb）和ND3基因使用TAG终止密码子外，PCG起始密码子为ATG、ATC、ATT和ATA，终止密码子为TAA。所有tRNA基因均折叠成预测的三叶草二级结构，反密码子环上具有必需的三联体，除了tRNA-Ser1（AGN），其双氢尿嘧啶（DHU）臂形成一个简单环。对半翅目线粒体基因组的系统发育分析聚类到科级水平，并支持半翅目异翅亚目的五个超科的单系性。缘蝽超科和红蝽超科是姐妹群，而缘蝽科和姬缘蝽科是红蝽科的姐妹群。这些分析为异翅亚目昆虫的线粒体基因组学和进化关系提供了见解。
