Zhou Jianshe, Zhang Chi, Wang Wanliang, Pan Yingzi, Zeng Benhe, Zhou Chaowei, Lei Luo, Li Baohai
Fisheries Research Institute, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet, People's Republic of China.
Department of Aquaculture, Southwest University Rongchang Campus, Chongqing, People's Republic of China.
Mitochondrial DNA B Resour. 2017 Sep 8;2(2):623-624. doi: 10.1080/23802359.2017.1372702.
The , a new recorded species in Lange Lake, was grouped into genus in Schizothoracinae, and had the rare quantity and limited resources on biology and genetics, especially in the mitochondrion. In this study, the complete mitochondrial sequence of was assembled and phylogenetic relationships with other species in Cyprinidae were analyzed. The whole mitochondrial sequence was 16,864 bp in length, which contained two control regions (D-loop regions), two rRNA genes (12S and 16S rRNA), 13 protein-coding genes and 22 tRNA genes. The D-loop region was separated by . The 12S rRNA and 16S rRNA located between and and were separated by . The 13 mRNAs had three start codons, five termination codons and four overlap regions. The 22 tRNA scattered among the whole mitochondrion, and they were range from 66 ( ) to 76 ( and ) in length. To further explore the phylogenetic relationship of the , we constructed the phylogenetic tree and verified that the was a part of genus and had closer relationship with and was independent from other species of Schizothoracinae, Barbinae and Labeoninae in Cyprinidae. This study provided the valuable evidence on phylogenetic relationship of the at the molecular level and essential resource for further research on this species.
该物种是朗格湖新记录物种，被归入裂腹鱼亚科的属中，其生物学和遗传学方面的数量稀少且资源有限，尤其是在线粒体方面。在本研究中，组装了该物种的完整线粒体序列，并分析了其与鲤科其他物种的系统发育关系。线粒体全序列长度为16,864 bp，包含两个控制区（D-loop区）、两个rRNA基因（12S和16S rRNA）、13个蛋白质编码基因和22个tRNA基因。D-loop区由 隔开。12S rRNA和16S rRNA位于 和 之间，间隔为 。13个mRNA有三个起始密码子、五个终止密码子和四个重叠区。22个tRNA分散于整个线粒体中，长度范围从66（ ）到76（ 和 ）。为进一步探究该物种的系统发育关系，构建了系统发育树，验证了该物种是属的一部分，与 关系更近，且独立于鲤科裂腹鱼亚科、鲃亚科和野鲮亚科的其他物种。本研究在分子水平上为该物种的系统发育关系提供了有价值的证据，也是对该物种进一步研究的重要资源。
