Funk Erik R, Adams Alexander N, Spotten Sarah M, Van Hove Roxanne A, Whittington Kristina T, Keepers Kyle G, Pogoda Cloe S, Lendemer James C, Tripp Erin A, Kane Nolan C
Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, CO, USA.
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO, USA.
Mitochondrial DNA B Resour. 2018 Feb 28;3(1):305-308. doi: 10.1080/23802359.2018.1445485.
Known colloquially as 'Old Man's Beard', is a genus of lichenized Ascomycete fungi characterized by having a fruticose growth form and cartilaginous central axis. The complete mitochondrial genomes of , , , , and were sequenced using Illumina data and then assembled . These mitogenomes ranged in size from 52,486 bp () to 94,464 bp (). All were characterized by having high levels of intronic and intergenic variation, such as ORFs that encode proteins with homology to two homing endonuclease types, LAGLIDADG and GIY-YIG. Genes annotated within these mitogenomes include 14 protein-coding genes, the large and small ribosomal subunits (LSU and SSU), and 23-26 tRNAs. Notably, the gene was absent from each genome. Genomic synteny was highly conserved across the five species. Five conserved mitochondrial genes (, , , , and ) were used to infer a best estimate maximum likelihood phylogeny among these five and other relatives, which yielded relationships consistent with prior published phylogenies.
通常被俗称为“老人须”,是一种地衣化子囊菌纲真菌,其特征是具有枝状生长形态和软骨状中轴线。使用Illumina数据对[物种名称1]、[物种名称2]、[物种名称3]、[物种名称4]和[物种名称5]的完整线粒体基因组进行测序,然后进行组装。这些线粒体基因组大小从52,486 bp([物种名称1])到94,464 bp([物种名称5])不等。所有这些基因组的特征都是内含子和基因间变异水平较高,例如存在编码与两种归巢内切酶类型(LAGLIDADG和GIY - YIG)具有同源性的蛋白质的开放阅读框(ORF)。这些线粒体基因组中注释的基因包括14个蛋白质编码基因、大核糖体亚基和小核糖体亚基(LSU和SSU)以及23 - 26个tRNA。值得注意的是,每个基因组中都没有[基因名称]基因。这五个物种的基因组同线性高度保守。使用五个保守的线粒体基因([基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]和[基因名称5])来推断这五个[物种名称]及其其他亲属之间的最佳估计最大似然系统发育树,其产生的关系与先前发表的系统发育树一致。