台湾泥鳅（亚种）（鳅科）的完整线粒体基因组
Complete mitochondrial genome of Taiwanese loach, ssp. (Cobitinae).
作者信息
Liang Xia, Zhu Daoyu, Li Xiaodong, Cai Kejun, Zhang Haili, Zhang Guosong
机构信息
Key Laboratory for Physiology Biochemistry and Application, School of Agriculture and Bioengineering, Heze University, Heze, Shandong, China.
School of Chemistry and Bioengineering, Hechi University, Yizhou, Guangxi, China.
出版信息
Mitochondrial DNA B Resour. 2018 Mar 13;3(1):340-341. doi: 10.1080/23802359.2018.1450665.
Taiwanese loach was bred in Formosa and then widely cultivated in China. Because of overfishing and environmental pollution, the number of wild Taiwanese loach has been sharply decreased in these years. Also the taxonomic status of Taiwanese loach is still unclear. In this study, the complete mitochondrial genome of Taiwanese loach was obtained by PCR. The genome is 16,569 bp in length, including 2 ribosomal RNA genes, 13 proteins-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition is 29.5% for A, 27.5% for T, 26.4% for C, and 16.6% for G. The sequence is a slight A + T bias of 57.0%. By analyzing phylogenetic analysis and BLAST, the similarity to Paramisgurnus was >99%, we dare to speculate that the Taiwanese loach cultivated in China was a subspecies of . Mitogenome information from this study could be a useful basis for conservation and phylogenetics of Taiwanese loach, ssp.
台湾泥鳅原产于台湾，后在中国广泛养殖。由于过度捕捞和环境污染，近年来野生台湾泥鳅数量急剧减少。此外，台湾泥鳅的分类地位仍不明确。在本研究中，通过聚合酶链反应（PCR）获得了台湾泥鳅的完整线粒体基因组。该基因组长度为16,569 bp，包括2个核糖体RNA基因、13个蛋白质编码基因、22个转运RNA基因和一个非编码控制区，其基因组成和顺序与其他脊椎动物报道的大多数相似。序列分析表明，总体碱基组成为：A占29.5%，T占27.5%，C占26.4%，G占16.6%。该序列存在轻微的A+T偏向，为57.0%。通过系统发育分析和BLAST分析，与大鳞副泥鳅的相似度>99%，我们大胆推测在中国养殖的台湾泥鳅是大鳞副泥鳅的一个亚种。本研究的线粒体基因组信息可为台湾泥鳅（大鳞副泥鳅亚种）的保护和系统发育学提供有用的依据。
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