太湖天然二倍体泥鳅的完整线粒体基因组
Complete mitochondrial genome of natural diploid loaches from the Taihu Lake.
作者信息
Zhang Guosong, Zhu Daoyu, Li Xiaodong, Liang Xia, Cai Kejun, Zhang Haili, Zhang Guisheng
机构信息
School of Agriculture and Bioengineering, Heze University, Heze, Shandong, China.
Chemistry and Bioengineering School, Hechi University, Yizhou, Guangxi, China.
出版信息
Mitochondrial DNA B Resour. 2018 May 11;3(2):566-567. doi: 10.1080/23802359.2018.1467227.
is a highly valued, aquaculture-relevant food fish in East Asian countries. Because of overfishing and environmental pollution, the number of wild Taihu loach has been sharply decreased in these years. In this study, the complete mitochondrial genome of Taihu loach was obtained by PCR. The genome is 16,646 bp in length, including 2 ribosomal RNA genes. Thirteen proteins-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition is 29.8% for A, 28.2% for T, 25.7% for C, and 16.3% for G. The phylogenetic tree showed that Misgurnus family got together for one branch, which includes Taihu , and the other loaches had their own branches. Also the mitochondrial genome sequence of were aligned by BLAST, compared with Cobitinae the sequence similarity could reach >94%, and the similarity to Misgurnus was >99%.
在东亚国家是一种备受重视、与水产养殖相关的食用鱼类。由于过度捕捞和环境污染，近年来野生太湖泥鳅的数量急剧减少。在本研究中，通过聚合酶链式反应（PCR）获得了太湖泥鳅的完整线粒体基因组。该基因组长度为16,646碱基对（bp），包括2个核糖体RNA基因、13个蛋白质编码基因、22个转运RNA基因和一个非编码控制区，其基因组成和顺序与其他脊椎动物报道的大多数情况相似。序列分析表明，总体碱基组成为：腺嘌呤（A）占29.8%，胸腺嘧啶（T）占28.2%，胞嘧啶（C）占25.7%，鸟嘌呤（G）占16.3%。系统发育树显示，泥鳅科聚为一个分支，其中包括太湖泥鳅，其他泥鳅各自有分支。此外，通过BLAST对[此处原文缺失物种名]的线粒体基因组序列进行比对，与花鳅亚科相比，序列相似性可达>94%，与泥鳅属的相似性为>99%。
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