Takahashi Jun-Ichi, Deowanish Sureerat, Okuyama Hisashi
Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan.
Department of Biology, Chulalongkorn University, Bangkok, Thailand.
Mitochondrial DNA B Resour. 2018 Mar 14;3(1):350-353. doi: 10.1080/23802359.2018.1450672.
The dwarf honeybees and inhabit the bush and forests of continental Asia and north Africa and some islands of Sundaland and the Philippines. We analysed, for the first time, the complete mitochondrial genomes of two dwarf honeybee species from Thailand using next-generation sequencing. Each mitochondrial genome was a circular and approximately 17 kbp molecule that included 13 protein-coding genes (PCGs), 22 tRNA genes, and two ribosomal RNA genes, along with one A + T-rich control region, besides three tRNA- () repeats. The AT content values of the mitochondrial genomes of and were 86.28% and 85.73%, respectively. The 1150 mutation sites in 13 PCGs differing between and in Thailand were evenly distributed throughout their mitochondrial genomes. The phylogenetic relationship, inferred using 13 PCGs, was consistent with that reported in previous studies, which predicted a sister relationship between and .
矮蜜蜂栖息于亚洲大陆和北非的灌木丛及森林,以及巽他群岛和菲律宾的一些岛屿。我们首次使用下一代测序技术分析了来自泰国的两种矮蜜蜂的完整线粒体基因组。每个线粒体基因组都是一个环状且约17千碱基对的分子,除了三个tRNA-()重复序列外,还包括13个蛋白质编码基因(PCGs)、22个tRNA基因和两个核糖体RNA基因,以及一个富含A+T的控制区。和的线粒体基因组的AT含量值分别为86.28%和85.73%。泰国的和在13个PCGs中的1150个突变位点在其线粒体基因组中均匀分布。使用13个PCGs推断的系统发育关系与先前研究报道的一致,该研究预测和之间是姐妹关系。